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  -人氣指數 119   -發佈時間 2017-12-05  
 
  BioVision_Fast and Reproducible Labeling of proteins and antibodies with different probes!!  
 
 

 

 

Fast and Reproducible Labeling of Proteins and Antibodies with Different Probes!!

Hello Customer,

BioVision’s EZLabel™ Protein and Antibody labeling kits provides an easy way to label proteins and antibodies with different probes in a user-friendly spin column format. These kits provide fast, stable and reproducible labeling of FITC, Cy3, Cy5 and Biotin to up to 1 mg of target. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target protein or antibody. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of protein or antibody per reaction. Labeled proteins and antibodies can be directly used for multiple downstream applications including ELISA, Western blot, Immunohistochemistry, Immunoassays, Flow cytometry and Immunofluorescence.

Key Features   

·        Simple: available in spin column format

·        Fast and efficient: takes 1-2 hrs. from start to finish

·        Direct applications: labeled conjugates can directly be used for subsequent use in different applications

·        Convenient: easy one-step labeling & separation method to label larger amounts of proteins and antibodies

Figures: SEC chromatogram of an Anti-BSA IgG (A) and BSA (B) labeled with Biotin (Cat. No. K834 and K835 respectively) using a Superdex 200 HR 10/30 column at 0.5 ml/min. in 50 mM Tris and 0.25 M NaCl pH 7.5. The absorbance was monitored at 280 nm. The spin column format ensured that the purification of antibody or protein was fast and there was no unreacted Biotin left after the antibody or protein was purified according to the kit protocol.

Check Out BioVision’s Antibody and Protein Labeling Kits:

Probe

Product Name

Cat. No.

Size

Biotin

EZLabelTM Antibody Biotin Labeling Kit

K834

5 x 1 mg

EZLabelTM Protein Biotin Labeling Kit

K835

5 x 1 mg

Cy3

EZLabelTM Antibody Cy3 Labeling Kit

K836

5 x 1 mg

EZLabelTM Protein Cy3 Labeling Kit

K837

5 x 1 mg

Cy5

EZLabelTM Antibody Cy5 Labeling Kit

K838

5 x 1 mg

EZLabelTM Protein Cy5 Labeling Kit

K839

5 x 1 mg

FITC

EZLabelTM Antibody FITC Labeling Kit

K831

5 x 1 mg

EZLabelTM Protein FITC Labeling Kit

K832

5 x 1 mg

 

Related Products:

 

 

Product Name

Cat. No.

Size

EZDetectTM Polyubiquitin Probe (Biotin Conjugated)

6569

250 μl

Gelatin-Fluorescein (FITC) Conjugate

M1303

1 mg, 5 x 1 mg

Collagen-Fluorescein (FITC) Conjugate

M1304

1 mg, 5 x 1 mg

PE-Cy5 (Phycoerythrin-Cy5 Conjugate)

7850

250 μg

Biotin Quantitation Kit (Colorimetric)

K811

100 assays

Protein A/G-Biotin

6506

100 μg, 1 mg

Protein G-Biotin

6512

100 μg, 1 mg