Alomone – Ion Channels | News & Updates

Alomone – Ion Channels | News & Updates

2019.01.27

  

Jan 24, 2019

 

Products for Immuno-Colocalization

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IHC) and immunocytochemistry (ICC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IHC and ICC.

Toxins conjugated to ATTO-fluorescent dyes: These labeled toxins can be used with any antibody to obtain highly specific channel localization.

Click here to view our complete line of immuno-colocalization products.

Guinea pig Anti-NaV1.5 (SCN5A) Antibody
(#AGP-008)

Immunohistochemical staining of rat heart frozen sections:

AGP-008

Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#AGP-008). NaV1.5 staining (red) appears in cardiomyocytes and in intercalated discs.

Anti-P2X1 Receptor (extracellular)-ATTO-488 Antibody (#APR-022-AG)

Immunohistochemical staining of mouse cerebellum:

APR-022-AG

Anti-P2X1 Receptor (extracellular)-ATTO-488 Antibody (#APR-022-AG). P2X1 labeling (green) appears in fine processes in the molecular layer and in Purkinje cells.

Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B) & Anti-KV1.5-ATTO-550 Antibody (#APC-004-AO)

Immuno-colocalization of KV1.3 and KV1.5 in mouse cerebellum:

APC-101-B

Immunohistochemical staining of mouse perfusion-fixed frozen brain sections using Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B) (1:400), and Anti-KV1.5-ATTO-550 Antibody (#APC-004-AO), (1:60). KV1.3 staining (green) and KV1.5 labeling (red) are co-localized in the soma of Bergmann glia. In the molecular layer, the distribution of KV1.5 is diffuse, unlike the discrete staining for KV1.3 in glial processes.

Products for Live Cell Flow Cytometry

We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.

With Alomone Labs extracellular antibodies:

No need for cell permeabilization and fixation

Cell surface detection of proteins

Anti-TRPV2 (extracellular)-FITC Antibody, (#ACC-039-F)

Direct flow cytometry analysis of intact living RBL cells:

alm-051

___ Unstained cells.
___ Cells + Anti-TRPV2 (extracellular)-FITC Antibody, (#ACC-039-F).

Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001)

Indirect flow cytometry analysis of Jurkat living cells:

azc-001

___ Unstained cells.
___ Cells + Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001), (5-10 µg/1×106 cells).

Products for Live Cell Imaging (LCI)

Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.

Click here to view our complete line of LCI products.

Mouse Anti-KCa3.1 (SK4) (extracellular) Antibody (#ALM-051)

Immunocytochemical staining of live intact human LN-CaP prostate carcinoma cells:

alm-051

Extracellular staining of cells with Mouse Anti-KCa3.1 (SK4) (extracellular) Antibody (#ALM-051), (red).

Anti-TRPA1 (extracellular) Antibody
(#ACC-037)

Immunocytochemical staining of intact living rat PC12 cells:

acc-037

Extracellular staining of cells using Anti-TRPA1 (extracellular) Antibody (#ACC-037), (1:50), (red).

Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004)

Expression of GABA(A) α6 receptor in rat PC12 cells:

AGA-004

Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. Extracellular staining of cells using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004), (1:100), (red).

New & Noteworthy

Selective & Potent NaV1.7 Channel Blockers

NaV1.7-Compound 36 (#CMN-003)

 

bioassay

CMN-003

Purity: >99% (HPLC)

Target: Blocker of NaV1.7 channels

Alomone Labs NaV1.7-Compound 36 blocks NaV1.7 channels expressed in Xenopus oocytes:

CMN-003

A. Representative time course of NaV1.7-Compound 36 (#CMN-003) inhibition of NaV1.7 channel peak currents. Membrane potential was held at -100 mV. Current was elicited by a 100 ms voltage step to 0 mV every 10 sec and inhibited by 10 µM and 50 µM NaV1.7-Compound 36 application (horizontal bars). B. Superimposed traces of NaV1.7 current upon application of control and of 10 µM and 50 µM NaV1.7-Compound 36, as indicated.

PF-05241328 (#P-345)

 

bioassay

P-345

Purity: >99%

CAS No.: 1387633-03-5

Target: Blocker of NaV1.7 channels

Alomone Labs PF-05241328 blocks NaV1.7 channels expressed in Xenopus oocytes:

P-345

A. Representative time course of PF-05241328 (#P-345) inhibition of NaV1.7 channel current. Membrane potential was held at -100 mV. Current was elicited by a 100 ms voltage step to 0 mV every 10 sec and inhibited by 10 µM and 50 µM PF-05241328 application (horizontal bars). B. Superimposed traces of NaV1.7 current upon application of control and of 10 µM and 50 µM PF-05241358, as indicated.

PF-05186462 (#P-365)

 

bioassay

P-365

Purity: >96% (HPLC)

CAS No.: 1235406-03-7

Target: Blocker of NaV1.7 channels

Alomone Labs PF-05186462 blocks NaV1.7 channels expressed in Xenopus oocytes:

P-365

A. Representative time course of PF-05186462 (#P-365) inhibition of NaV1.7 channel currents. Membrane potential was held at -100 mV. Current was elicited by a 100 ms voltage step to 0 mV every 10 sec and inhibited by 1 µM and 5 µM PF-05186462 application (horizontal bars). B. Superimposed traces of NaV1.7 current upon application of control and of 1 µM and 5 µM PF-05186462, as indicated.

Selective & Potent ASIC3 Channel Potentiators

Cono-RFamides are a subclass of conotoxins, peptide toxins originally isolated from Conus species. They are characterized by a very short sequence and are devoid of disulphide bridges. Cono RPRF-amide (#STC-170), Cono VGRPRF-amide (#STC-172), and Cono AIVGRPRF-amide (#STC-174) have all been originally isolated from Conus textile venom and are potent activators of ASIC3 channels.

 

Cono RPRF-amide (#STC-170)

 

bioassay

stc-170

Purity: >98% (HPLC)

Target: Activator of ASIC3 channel

Alomone Labs Cono RPRF-amide positively modulates ASIC3 channels expressed in Xenopus oocytes:

stc-170

A. Representative time course of Cono RPRF-amide (#STC-170) potentiation of ASIC3 current. Membrane potential was held at -60 mV, current was elicited every 100 sec by a transient application of pH 5.5, and reversibly potentiated by a 60 sec application of 50 µM Cono RPRF-amide, as indicated. B. Superimposed traces of ASIC3 currents before (black) and following (green) application of 50 µM Cono RPRF-amide, which significantly extends the current following stimulation (arrow). Taken from the experiment in A.

Cono VGRPRF-amide (#STC-172)

 

bioassay

stc-172

Purity: >98% (HPLC)

Target: Activator of ASIC3 channel

Alomone Labs Cono VGRPRF-amide positively modulates ASIC3 channels expressed in Xenopus oocytes:

TEL:+886-2-88663366   FAX:+886-2-28331789   MAIL:info@abscience.com.tw

11163台北市士林區文林路661巷1號1樓   1F., No.1, Ln. 661, Wenlin Rd., Shilin Dist., Taipei City 11163, Taiwan (R.O.C.)

亞旭生物科技股份有限公司版權所有   Copyright ©2020 ASIA BIOSCIENCE CO. LTD. All Rights Reserved.

back-Top
open-line

LINE 線上諮詢

亞旭-LINE-QRcode

掃瞄或手機直接點擊條碼

error: Content is protected !!