Alomone-Ion Channels | News & Updates

Alomone-Ion Channels | News & Updates

2018.03.31

 

News & Updates – Mar 21, 2018

 

Products for Immuno-Colocalization

 

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IH) and immunocytochemistry (IC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IH and IC.

Toxins conjugated to ATTO-fluorescent dyes: These labeled toxins can be used with any antibody to obtain highly specific channel localization.

Click here to view our complete line of immuno-colocalization products.

Guinea pig Anti-Kir3.2 (GIRK2)

AGP-013

Immunohistochemical staining of rat substantia nigra pars compacta (SNC): Guinea pig Anti-Kir3.2 (GIRK2) antibody (#AGP-013), (1:400). GIRK2 staining (green) appears in soma and dendrites of dopaminergic neurons.

Anti-Kir7.1 (extracellular)-ATTO-488

APC-125-AG

Immunohistochemical staining of paraffin-embedded rat kidney sections: Anti-Kir7.1 (extracellular)-ATTO-488 antibody (#APC-125-AG), (1:50). Kir7.1 staining (green) is present in convoluted tubules in the renal cortex.

Anti-SERCA1 & Guinea pig Anti-TRPV2 (extracellular)

AGP-033

Immuno-colocalization of SERCA1 and TRPV2 in mouse heart.

Immunohistochemical staining of mouse heart immersion-fixed, free floating frozen sections, using rabbit Anti-SERCA1 antibody (#ACP-011) (1:200) and Guinea pig Anti-TRPV2 (extracellular) antibody (#AGP-033), (1:200). Co-localization of SERCA1 and TRPV2 is observed in T tubules.

Products for Live Cell Flow Cytometry

We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.

With Alomone Labs extracellular antibodies:

No need for cell permeabilization and fixation

Cell surface detection of proteins

Anti-Kir2.1 (extracellular)

APC-159

Indirect flow cytometry of live intact human THP-1 monocytic leukemia cells:

___ Cells.
___ Cells + goat-anti-rabbit-Alexa-488.
___ Cells + Anti-Kir2.1 (extracellular) antibody (#APC-159), 3 µl + goat-anti-rabbit-AlexaFluor-488 secondary antibody.

Anti-P2X1 Receptor (extracellular)-FITC

apr-022-f

Direct flow cytometry of live intact human MEG-01 megakaryoblastic leukemia cells:

___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-P2X1 Receptor (extracellular)-FITC antibody (#APR-022-F), (5 µg antibody/0.5×106 cells).

Products for Live Cell Imaging (LCI)

Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.

Click here to view our complete line of LCI products.

Anti-NaV1.7 (extracellular)

asc-027

Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells: Extracellular staining of cells using Anti-Nav1.7 (extracellular) antibody (#ASC-027), (red).

Guinea pig Anti-AMPA Receptor 2 (ext.)

agp-073

Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells: Extracellular staining of cells using Guinea pig Anti-AMPA Receptor 2 (GluA2) (extracellular) antibody (#AGP-073), (red).

Anti-Pannexin 2 (extracellular)

acc-232

Immunocytochemical staining of live intact human U-87 MG glioblastoma cells: Extracellular staining of cells with Anti-Pannexin 2 (extracellular) antibody (#ACC-232), (1:50), (red).

New & Noteworthy

Specific ASIC3 Channel Potentiators

 

Cono-RFamides are a subclass of conotoxins, peptide toxins originally isolated from Conus species. They are characterized by a very short sequence and are devoid of disulphide bridges. Cono RPRF-amide (#STC-170), Cono VGRPRF-amide (#STC-172), and Cono AIVGRPRF-amide (#STC-174) have all been originally isolated from Conus textile venom and are potent activators of ASIC3 channels.

 

Cono RPRF-amide (#STC-170)

stc-170

stc-170

Alomone Labs Cono RPRF-amide (#STC-170) positively modulates ASIC3 channels expressed in Xenopus oocytes.

Cono VGRPRF-amide (#STC-172)

stc-172

stc-172

Alomone Labs Cono VGRPRF-amide (#STC-172) positively modulates ASIC3 channels expressed in Xenopus oocytes.

Cono AIVGRPRF-amide (#STC-174)

stc-174

stc-174

Alomone Labs Cono AIVGRPRF-amide (#STC-174) positively modulates ASIC3 channels expressed in Xenopus oocytes.

Potent & Selective P2X3 Receptor Antagonists

 

A-317491 sodium salt hydrate (#A-380)

Effective concentration: 50 nM – 50 µM

 

AF-353 (#A-375)

Effective concentration: 1 nM – 10 µM

 

RO-3 (#R-190)

Effective concentration: 50 nM – 50 µM

 

Ro-51 (#R-195)

Effective concentration: 0.2 nM – 0.2 µM

 

a-380

A-317491 sodium salt hydrate

Ion Channel Products in Featured Papers

Role of KV7 Channels in Coronary Arteries

The role of KV7 channels in vasodilation by hypoxia and the effect of diabetes on their expression and activity were investigated.

Electrophysiological recordings of left coronary arteries (LCA) versus right coronary arteries (RCA) showed that LCAs were more sensitive to KV7 blockers and activators. Indeed, immunocytochemical staining of rat smooth muscle cells from LCA and RCA using Anti-KV7.1 (KCNQ1) antibody (#APC-022) and Anti-KV7.5 antibody (#APC-155) showed that the expression of both channels is higher in LCA than in RCA (Figure 1). The physiological consequences of the differential expression of KV7 channels is also apparent under hypoxia and high glucose, which both show a decrease in LCA expression and an impairment of KV7 channels in LCA but not in RCA.

apc-022

Figure 1. Expression of KV7.1 and KV7.5 in rat smooth muscle cells.

Immunocytochemical staining of rat smooth muscle cells from LCA and RCA using Anti-KV7.1 (KCNQ1) antibody (#APC-022) and Anti-KV7.5 (KCNQ5) antibody (#APC-155) shows that the expression of both channels is higher in LCA than in RCA.

Adapted from Morales Cano, D. et al. (2015) with permission of the European Society of Cardiology.

 

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