Alomone labs_Ion Channels | News & Updates

Alomone labs_Ion Channels | News & Updates

2017.08.25

 

 

News & Updates – August 2017

 

Products for Immuno-Colocalization

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IH) and immunocytochemistry (IC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IH and IC.

Biotinylated neurotrophins: These labeled neurotrophins can be used with any antibody to obtain highly specific localization.

Click here to view our complete line of immuno-colocalization products.

Anti-Vesicular GABA Transporter-ATTO-594

AGT-005-AR

Immunohistochemical staining of rat frozen cerebellum sections: Anti-Vesicular GABA Transporter-ATTO-594 antibody (#AGT-005-AR), (1:100). VGAT staining (red) is most intensely expressed in the granule and pyramidal layer.

Anti-Kir4.1 (guinea pig)

AGP-012

Immunohistochemical staining of rat frozen brain sections: Anti-Kir4.1 guinea pig antibody (#AGP-012), (1:300). Kir4.1 staining (green) reveals Kir4.1 clusters in the molecular layer. GFAP staining is shown in red.

Anti-Vesicular Monoamine Transporter 2-ATTO-488 & Anti-TRPC3-ATTO-594

AMT-006-AG

Immuno-colocalization of VMAT2 and TRPC3 in rat brain

Immunohistochemical staining of perfusion-fixed frozen rat substancia nigra sections using Anti-Vesicular Monoamine Transporter 2-ATTO-488 antibody (#AMT-006-AG), (1:60), (green) and Anti-TRPC3-ATTO-594 antibody (#ACC-016-AR), (1:60), (red). Ubiquitous colocalization of VMAT2 and TRPC3 in cells of the substantia nigra pars compacta is observed.

Products for Live Cell Flow Cytometry

We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.

With Alomone Labs extracellular antibodies:

No need for cell permeabilization and fixation

Cell surface detection of proteins

Anti-Human Orai1 (extracellular)

acc-060

Indirect flow cytometry analysis of intact HL-60 cells:

___ Unstained cells.
___ Cells + Anti-Human Orai1 (extracellular) antibody (#ACC-060), (5-10 µg antibody/0.5-1 x 106 cells).

Anti-KCa1.1 (BKCa) (extracellular)

APC-151

Indirect flow cytometry analysis of live intact THP-1 (human acute monocytic leukemia cells) cell line:

___ Cells + goat-anti-rabbit-Cy5.
___ Cells + Anti-KCa1.1 (BKCa) (extracellular) antibody (#APC-151), (1:20) + goat-anti-rabbit-Cy5.

Products for Live Cell Imaging (LCI)

Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.

Click here to view our complete line of LCI products.

Anti-NBCe1 (extracellular)

ANT-075

Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells: Extracellular staining of cells with Anti-NBCe1 (extracellular) antibody (#ANT-075), (1:100), (red) merged with live view of cells.

Anti-TRPM8 (extracellular)

ACC-049

Immunocytochemical staining of intact living rat root dorsal root ganglion (DRG) cells: Extracellular staining of cells with Anti-TRPM8 (extracellular) antibody (#ACC-049), (1:500), (red).

Anti-TRPC6 (extracellular)

ACC-120

Expression of TRPC6 in rat PC12 cells

Immunocytochemical staining of intact living PC12 cells. Extracellular staining of cells with Anti-TRPC6 (extracellular) antibody (#ACC-120), (1:50), (red).

New & Noteworthy

Novel GluN2A Receptor Antagonist!

 

MPX-004 (#M-280) is a synthetic compound that acts as a potent and selective antagonist of GluN2A. It displays an IC50 value of 79 nM in HEK cells.

m-280

m-280

MPX-004

Alomone Labs MPX-004 inhibits NMDA receptor channels expressed in Xenopus oocytes. 

A. Time course of NR1/NR2A currents, elicited by transient stimulations with 200 µM glutamate and 200 µM glycine every 100 sec, while membrane potential was held at -80 mV. 100 nM MPX-004 (#M-280), applied for 7 min (bar), significantly inhibits current amplitude. B. Superimposed current traces from the recording shown in A, upon application of control and of 100 nM MPX-004 (as indicated).

Ion Channel Products in Featured Papers

AQP2 Excretion and Expression Decrease during Gentamicin Treatment

AQP2 could be a candidate for detecting kidney collecting duct dysfunction as a result of gentamicin treatment. Immunohistochemical staining of rat kidney sections using Anti-Aquaporin 2 antibody (#AQP-002) showed that AQP2 expression and excretion decrease following gentamicin treatment (Abdeen, A. et al. (2014) Am. J. Physiol. 307, F1227.).

AQP-002

Expression of AQP2 in rat kidney sections before and after gentamicin treatment.
Immunohistochemical staining of rat kidney sections using Anti-Aquaporin 2 antibody (#AQP-002) after GM treatment. Kidney sections from rats after 2 days of treatment with vehicle (control; A, D, and G) or GM (B, E, and H) or 8 days of treatment with GM (C, F, I, and J). Representative images of the cortex (A–C), outer medulla (D–F), and inner medulla (G–J) are shown. The box in I indicates the region of high magnification in J. Brown staining indicates the presence of AQP2.
Adapted from Abdeen, A. et al. (2014) with permission of the American Physiological Society.

Modulator Magazine

The Modulator is dedicated to hot topics and recent developments in ion channel research. In each issue, we present elegant and exciting new ways our products are being used in papers from reputable journals. 

Take a look at our latest Modulator issue.

Subscribe and receive a free printed copy!

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