Alomone_Ion Channels | News & Updates

Alomone_Ion Channels | News & Updates

2018.05.15

 

 

Products for Immuno-Colocalization

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IH) and immunocytochemistry (IC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IH and IC.

Toxins conjugated to ATTO-fluorescent dyes: These labeled toxins can be used with any antibody to obtain highly specific channel localization.

Click here to view our complete line of immuno-colocalization products.

Guinea pig Anti-K2P2.1 (TREK-1) Antibody

AGP-049

Immunohistochemical staining of perfusion-fixed frozen rat brain sections: Guinea pig Anti-K2P2.1 (TREK-1) Antibody (#AGP-049), (1:400), (red). TREK-1 staining appears in neurons of lateral substantia nigra.

Anti-Aquaporin 2-ATTO-550 Antibody

AQP-002-AO

Immunohistochemical staining of rat kidney paraffin embedded region: Anti-Aquaporin 2-ATTO-550 Antibody (#AQP-002-AO), (1:50), (red). Aquaporin 2 is detected in collecting ducts but not in thin segments of the loop of Henle.

Anti-Nicotinic Acetylcholine Receptor α4 (ext.) Antibody & Anti-Nicotinic Acetylcholine Receptor β2 (ext.)-ATTO-594 Antibody

anc-004

Immuno-colocalization of nAChRα4 and nAChRβ2 in mouse brain

Immunohistochemical staining of perfusion-fixed frozen mouse parietal cortex sections using Anti-Nicotinic Acetylcholine Receptor α4 (extracellular) Antibody (#ANC-004), (1:300), (green) and Anti-Nicotinic Acetylcholine Receptor β2 (extracellular)-ATTO-594 Antibody (#ANC-012-AR), (1:60), (red). Several cells expressing both receptors are observed.

Products for Live Cell Flow Cytometry

We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.

With Alomone Labs extracellular antibodies:

No need for cell permeabilization and fixation

Cell surface detection of proteins

Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody

azc-001

Indirect flow cytometry analysis of Jurkat living cells:

___ Unstained cells.
___ Cells + Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001), (5-10 µg/1×106 cells).

Anti-TRPV2 (extracellular)-FITC Antibody fffff fff

ACC-039-F

Direct flow cytometry analysis of intact living RBL cells:

___ Unstained cells.
___ Cells + Anti-TRPV2 (extracellular)-FITC Antibody (#ACC-039-F).

Products for Live Cell Imaging (LCI)

Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.

Click here to view our complete line of LCI products.

Anti-Sodium Leak Channel Non-Selective Protein (Nalcn) (extracellular) Antibody

asc-022

Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells:  Extracellular staining of cells using Anti-Sodium Leak Channel Non-Selective Protein (Nalcn) (extracellular) Antibody (#ASC-022), (1:50), (red).

Anti-K2P18.1 (TRESK) (extracellular) Antibody ff

apc-122

Immunocytochemical staining of living mouse/rat neuroblastoma x dorsal root ganglion neuron hybrid cell line (ND7/23): Extracellular staining of cells with Anti-K2P18.1 (TRESK) (extracellular) Antibody (#APC-122), (1:50), (red).

Anti-Kir2.1 (extracellular) Antibody

apc-159

Immunocytochemical staining of live intact rat RBL-2H3 basophilic leukemia cells: Extracellular staining of cells using Anti-Kir2.1 (extracellular) Antibody (#APC-159), (1:50), (green).

New & Noteworthy

A Potent and Selective NaV1.7 channel blocker

 

m3-Huwentoxin IV (#STH-102) is a highly pure, synthetic, and biologically active peptide toxin. It is a mutated variant of Huwentoxin IV, a peptide toxin originally isolated from the Chinese bird-eating spider Haplopelma schmitdi venom. m3-Huwentoxin IV is a significantly more potent NaV1.7 channel blocker, displaying an IC50 value of 0.4 nM.

 

m3-Huwentoxin IV (#STH-102)

sth-102

sth-102

Alomone Labs m3-Huwentoxin IV (#STH-102) blocks NaV1.7 channels expressed in Xenopus oocytes.

A Selective and Reversible ASIC1a Blocker

Mambalgin-1 (#STM-100) is a highly pure, synthetic, and biologically active peptide toxin. It was originally isolated from Dendroaspis polylepis polylepis (Black mamba) snake venom. Mambalgin-1 potently and selectively blocks ASIC1a channels.

 

Mambalgin-1 (#STM-100)

stm-100

stm-100

Alomone Labs Mambalgin-1 inhibits ASIC1a channels expressed in Xenopus oocytes.

A. Representative time course of Mambalgin-1 (#STM-100) inhibition of ASIC1a. Currents were elicited every 50 sec by transient application of pH 6, while membrane potential was held at -80 mV, and inhibited by 20 nM and 100 nM Mambalgin-1 (indicated by bars). B. Superimposed traces of ASIC1a currents upon application of control, 20 nM and 100 nM Mambalgin-1 (taken from the recording in A).

Ion Channel Products in Featured Papers

TRPV2 in Stress-Induced Ca2+ Influx

Duchenne muscular dystrophy (DMD) is caused by a lack in functional dystrophin, leading to the degeneration of skeletal muscle, cardiac failure and arrhythmias. At the molecular level, the lack of dystrophin expression causes the malfunction of stretch-activated channels, leading in turn to increased stress-induced Ca2+ influx.
TRPV2 channel was found to be involved in the increase in Ca2+ influx observed in cardiomyocytes isolated from the mouse model system for DMD. In diseased mice, immunocytochemical staining of cardiomyocytes using Alomone Labs Anti-TRPV2 (extracellular) Antibody (#ACC-039) showed that TRPV2 is translocated to the sarcolemma and is prominent along the T-tubules (Figure 1). In addition, blocking the channel using Anti-TRPV2 (extracellular) antibody protected the cells from stress-induced abnormal Ca2+ signals.

acc-039

Expression of TRPV2 in mouse cardiomyocytes.

Immunocytochemical staining of mouse cardiomyocytes. Staining of cells using Anti-TRPV2 (extracellular) Antibody (#ACC-039) (green), shows that TRPV2 is translocated to the sarcolemma along the T-tubules in cardiomyocytes from DMD mice (lower left panel).

Adapted from Lorin, C. et al. (2015) with permission of European Society of Cardiology.

back-Top
open-line

LINE 線上諮詢

亞旭-LINE-QRcode

掃瞄或手機直接點擊條碼

error: Content is protected !!