Alomone_Ion Channels | News & Updates

Alomone_Ion Channels | News & Updates

2018.09.25

 

Sep 25, 2018

 

Products for Immuno-Colocalization

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IHC) and immunocytochemistry (ICC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IHC and ICC.

Toxins conjugated to ATTO-fluorescent dyes: These labeled toxins can be used with any antibody to obtain highly specific channel localization.

Click here to view our complete line of immuno-colocalization products.

Anti-IP3 Receptor-2-ATTO-594 Antibody (#ACC-116-AR)


Immunohistochemical staining of rat spinal cord:

ACC-116-AR

Anti-IP3 Receptor-2-ATTO-594 Antibody (#ACC-116-AR), (1:60). IP3 Receptor-2 immunoreactivity (red) appears in neuronal soma and processes.

Anti-GIRK2 (Kir3.2) Antibody (#APC-006) & Guinea pig Anti-K2P2.1 (TREK-1) Antibody (#AGP-049)

Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections:

APC-006

Anti-GIRK2 (Kir3.2) Antibody (#APC-006), (1:400) and Guinea pig Anti-K2P2.1 (TREK-1) Antibody (#AGP-049), (1:120). Co-localization of TREK-1 and GIRK2 is observed mainly in the SNC region.

Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001) & Anti-CALHM1-ATTO-594 Antibody (#ACC-101-AR)

Immuno-colocalization of GluN1 and CALHM1 in mouse hippocampal CA1 region:

ACC-101-AR

Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:200), (green) and Anti-CALHM1-ATTO-594 Antibody (#ACC-101-AR), (1:60), (red). Colocalization of GluN1 and CALHM1 in pyramidal neurons is clearly detected.

Products for Live Cell Flow Cytometry

We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.

With Alomone Labs extracellular antibodies:

No need for cell permeabilization and fixation

Cell surface detection of proteins

Mouse Anti-KCa3.1 (SK4) (extracellular) Antibody (#ALM-051)

Indirect flow cytometry analysis of live intact THP-1 (human acute monocytic leukemia cells) cell line:

ALM-051

___ Cells + Goat-anti-mouse-Cy5.
___ Cells + Mouse IgM isotype control+ Goat-anti-mouse-Cy5.
___ Cells + Mouse Anti-KCa3.1 (SK4) (extracellular) Antibody (#ALM-051), (1:20) + Goat-anti-mouse-Cy5.

Anti-P2X1 Receptor (extracellular)-FITC Antibody (#APR-022-F)


Direct flow cytometry of live intact human MEG-01 megakaryoblastic leukemia cells:

APR-022-F_

___ Cells.
___ Cells + rabbit IgG isotype control-FITC
___ Cells + Anti-P2X1 Receptor (extracellular)-FITC Antibody (#APR-022-F), (5 µg antibody/0.5×106 cells).

Products for Live Cell Imaging (LCI)

Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.

Click here to view our complete line of LCI products.

Anti-GABA(A) ε Receptor (extracellular) Antibody (#AGA-015)

Immunocytochemical staining of intact living human U-87 MG cells:

AGA-015

Extracellular staining of cells using Anti-GABA(A) ε Receptor (extracellular) Antibody (#AGA-015), (1:25), (red).

Guinea pig Anti-TRPV2 (extracellular) Antibody (#AGP-033)

Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells:

AGP-033

Extracellular staining of cells using Guinea pig Anti-TRPV2 (extracellular) Antibody (#AGP-033), (1:100), (green).

Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025)

Expression of Orai1 in HEK-293 transfected cells:

ALM-025

Immunocytochemical staining of intact living HEK-293 cells expressing Orai1. Extracellular staining of HEK-293 cells transfected with Orai1 using Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025), (red).

New & Noteworthy

Super Potent GABA(A) Receptor Allosteric Modulators

MmTX1 (#STM-550) and MmTX2 (#STM-600) are peptide toxins originally isolated from Micrurus mipartitus (Red-tailed coral snake) venom. They act as potent allosteric modulators of GABA(A) receptors at subnanomolar concentrations. Both allosterically increase GABA(A) receptor susceptibility to agonistic actions, thereby potentiating receptor opening and desensitization. MmTX1 and MmTX2 may be a priceless tool in evoking seizures for testing novel antiepileptic drugs or as lead molecules for designing therapeutics that modulate GABAA receptor activity.

 

Alomone Labs MmTX1 and MmTX2 are highly pure, synthetic, and biologically active peptide toxins.

 

MmTX1 (#STM-550)

STM-550

Alomone Labs MmTX1 modulates GABA(A) receptors:

STM-550

A. Representative time course of GABA(A) α1/β2 current activated at a holding potential of -80 mV by 100 nM Muscimol hydrobromide (#M-240) applications (black bars), and modulated by co-application of 200 nM MmTX1 (#STM-550), as indicated (green bar). A significant modulation of receptor desensitization and reactivation rates is observed. B. Superimposed traces of GABA(A) receptor currents upon application of 100 nM Muscimol (black) or co-application of 100 nM Muscimol and 200 nM MmTX1 (green). Taken from the recording in A.

MmTX2 (#STM-600)

STM-600

Alomone Labs MmTX2 modulates GABA(A) receptors:

STM-600

A. Representative time course of GABA(A) α1/β2 currents activated at a holding potential of -80 mV with 50 nM Muscimol hydrobromide (#M-240) application (black bars), and modulated by co-application of 200 nM MmTX2 (#STM-600), as indicated (green bar). A significant modulation of receptor desensitization rate is observed. B. Superimposed traces of GABA(A) currents upon application of 50 nM Muscimol hydrobromide (black) or co-application of 50 nM Muscimol hydrobromide and 200 nM MmTX2 (green). Taken from the recording in A.

A Selective and Potent Activator of NaV1.1 Channel

Hm1a (#STH-601)

 

Alomone Labs Hm1a is a peptide toxin originally isolated from Heteroscodra maculate tarantula venom. It is a selective and specific activator of Nav1.1. Hm1a inhibits human Nav1.1 channels inactivation expressed in Xenopus oocytes with EC50 value of 38 ± 6 nM.

 

STH-601

Alomone Labs Hm1a enhances the current of NaV1.1 channels:

STH-601

A. Representative time course of Hm1a (#STH-601) effect on the normalized area of NaV1.1 channels current. Membrane potential was held at -90 mV, current was elicited by a 100 ms voltage step to -10 mV every 10 sec and was significantly enhanced by the application of 250 nM Hm1a (green). B. Superimposed traces of NaV1.1 current after application of control (black) and of 250 nM Hm1a (green), taken from the recording in A.

Ion Channel Products in Featured Papers

Downregulation of TRPV4 in Diabetes

TRPV4 channel functional expression is downregulated as a result of diabetes complication and leads to loss of vision. Immunohistochemical staining of rat retinal sections using Anti-TRPV4 Antibody (#ACC-034) shows that the channel is mostly expressed in the endothelium of retinal microvessels (Figure 1A). Sections from diabetic animals show a decrease of about 70% in TRPV4 expression when compared to normal sections (Figure 1C). The specificity of the antibody was demonstrated using the control antigen (Figure 1B).

Overall, the study raises TRPV4 function as a possible target in diabetic retinopathy.

 

Figure 1: Downregulation of TRPV4 in rat retinal microvascular endothelium:

acc-034

Immunohistochemical staining of rat retinal sections using Anti-TRPV4 Antibody (#ACC-034). A. TRPV4 expression (green) is mostly detected in the endothelium of retinal microvessels. B. Specificity of the antibody is depicted by showing loss of TRPV4 staining when pre-incubating with the control peptide antigen. C. Significant decrease in TRPV4 expression is observed in retina section from diabetic animals.

Adapted from Monaghan, K. et al. (2015) with kind permission of Curtis, T.M., Centre for Experimental Medicine, Queen’s University of Belfast.

 

Stay tuned for more exciting products and check out our New Products page!

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