alomone_Ion Channels | News & Updates

alomone_Ion Channels | News & Updates

2017.10.11

 

Products for Immuno-Colocalization

 

Products for Immuno-Colocalization

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IH) and immunocytochemistry (IC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IH and IC.

Biotinylated neurotrophins: These labeled neurotrophins can be used with any antibody to obtain highly specific localization.

Anti-KV1.3 (extracellular) (guinea pig)

agp-005

Immunohistochemical staining of perfusion-fixed frozen mouse brain sections: Anti-KV1.3 (extracellular) guinea pig antibody (#AGP-005), (1:100). KV1.3 staining (red) is detected in Bergmann glia outlines. DAPI is used as the counterstain (blue).

Anti-P2X3 Receptor-ATTO-594

apr-016-ar

Immunohistochemical staining of rat dorsal root ganglion (DRG): Anti-P2X3 Receptor-ATTO-594 antibody (#APR-016-AR). P2X3 staining (red) in DRG neurons merged with DAPI staining (blue).

Anti-Rat TRPV1 (extracellular)-ATTO-488 & Anti-NaV1.8-ATTO-594

ACC-029-AG

Immuno-colocalization of TRPV1 and NaV1.8 in rat DRG

Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-Rat TRPV1 (extracellular)-ATTO-488 antibody (#ACC-029-AG), (green), (1:60) and Anti-NaV1.8-ATTO-594 antibody (#ASC-016-AR), (red), (1:60). Partial co-localization between TRPV1 and NaV1.8 channels is observed.

Products for Live Cell Flow Cytometry

We put in tremendous effort in designing antibodies that recognize extracellular domains of proteins.

With Alomone Labs extracellular antibodies:

No need for cell permeabilization and fixation

Cell surface detection of proteins

Anti-Human Orai1 (extracellular)

acc-060

Indirect flow cytometry analysis of intact Jurkat cells.

___ Unstained cells.
___ Cells + Anti-Human Orai1 (extracellular) antibody (#ACC-060), (5-10 µg antibody/0.5-1 x 106 cells).

Anti-KV11.1 (HERG) (extracellular)

apc-109

Indirect flow cytometry analysis of human chronic myelogenous leukemia (K562) cells:

___ Unstained cells + goat-anti-rabbit-FITC.
___ Cells + Anti-KV11.1 (HERG) (extracellular) antibody (#APC-109) + goat-anti-rabbit-FITC.

Products for Live Cell Imaging (LCI)

Alomone Labs is committed to developing cutting edge reagents for visualizing ion channels in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various ion channels. Below are new antibodies ideal for LCI.

Anti-pan ASIC (extracellular)

asc-031

Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells: Extracellular staining of cells with Anti-pan ASIC (extracellular) antibody (#ASC-031), (green).

Anti-Pannexin 2 (extracellular)

acc-232

Immunocytochemical staining of live intact human U-87 MG glioblastoma cells: Extracellular staining of cell with Anti-Pannexin 2 (extracellular) antibody (#ACC-232), (red).

Anti-GABA(A) α4 Receptor (extracellular)

aga-008

Expression of GABA(A) α4 Receptor in rat PC12 cells

Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. Extracellular staining of cells using Anti-GABA(A) α4 Receptor (extracellular) antibody (#AGA-008), (red).

New & Noteworthy

Novel GABA(A) Receptor Antagonist

 

GABA(A)-Compound 1b (#CMG-004) is a synthetic compound that acts as an antagonist of GABA(A) α5-containing receptors, displaying an IC50 value of 201 nM (Ling, I. et al. (2015) Eur. J. Pharmacol. 764, 497.).

In our bioassay, we show partial inhibition of GABA(A) α1/β2 currents in 2 µM GABA(A)-Compound 1b.

cmg-004

cmg-004

GABA(A)-Compound 1b

Alomone Labs GABA(A)-Compound 1b inhibits GABA(A) receptors expressed in Xenopus oocytes.

Representative time course of GABA(A) α1/β2 current, activated by a continuous application (top dotted line) of 0.1 µM γ-aminobutyric acid (#G-110), and partially inhibited by 2 µM GABA(A)-Compound 1b (#CMG-004), as indicated (bar), at a holding potential of -60 mV.

Ion Channel Products in Featured Papers

TRPV1 Plasma Membrane Localization in Peptidergic Nociceptors is Dependent on αCGRP

Immunocytochemical staining of mouse DRG neurons using Anti-TRPV1 antibody (#ACC-030) shows that TRPV1 co-localizes with CGRP in large dense core vesicles (LDCVs) (Devesa, I. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. pii: 201420252.).

acc-030

TRPV1 co-localizes with CGRP in mouse DRG neurons.

Immunocytochemical staining of mouse DRG neurons using Anti-TRPV1 antibody (#ACC-030). TRPV1 (green) co-localizes with CGRP (red) in large dense core vesicles (LDCVs).
Adapted from Devesa, I. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. pii: 201420252.

Modulator Magazine

The Modulator is dedicated to hot topics and recent developments in ion channel research. In each issue, we present elegant and exciting new ways our products are being used in papers from reputable journals. 

Take a look at our latest Modulator issue.

 

 

 

 

 

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