Alomone_TRP Channel | Novel Antibodies for Immuno-Colocalization

Alomone_TRP Channel | Novel Antibodies for Immuno-Colocalization

2018.09.07

 

Sep 04, 2018

Novel-TRP-Channel-Antibodies_IC

 

Alomone Labs has developed innovative product lines for studying protein-protein interaction and/or protein co-localization:

 

Conjugated Rabbit Polyclonals

Primary antibodies conjugated to ATTO-fluorescent dyes or Biotin can be used in immunohistochemistry (IH) and immunocytochemistry (IC) with same species antibodies.

Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR)

Anti-TRPM2-ATTO-594 Antibody (#ACC-043-AR)

Anti-TRPML3-ATTO-594 Antibody (#ACC-083-AR)

Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG)

Anti-TRPV3 (extracellular)-ATTO-633 Antibody (#ACC-033-FR)

Anti-TRPV3 (extracellular)-Biotin Antibody (#ACC-033-B)

Anti-TRPV4-ATTO-550 Antibody (#ACC-034-AO)

 

Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR)

 

Colocalization of TRPC6 and TRPC3 in rat cerebellum:

 

ACC-016-AR

Immunohistochemical staining of rat cerebellum frozen section using Guinea pig Anti-TRPC6 Antibody (#AGP-002) and rabbit Anti-TRPC3-ATTO-594 Antibody (#ACC-016-AR). A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section as in A, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates co-localization in Purkinje cells and molecular layer.

 

Anti-TRPM2-ATTO-594 Antibody (#ACC-043-AR)

 

Expression of TRPM2 in rat lung:

ACC-043-AR

Immunohistochemical staining of rat lung frozen sections using Anti-TRPM2-ATTO-594 Antibody (#ACC-043-AR), (1:50). A. TRPM2 staining is specific for respiratory epithelium in the bronchi. B. Merged with bright field.

 

 

Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG)

 

Immuno-colocalization of TRPV1 and mGluR5 in rat DRG:

ACC-029-AG

Immunohistochemical staining of rat dorsal root ganglion using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG), (1:60) and Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR), (1:60).  A. TRPV1 staining (green). B. mGluR5 staining of the same section (red). C.  Merge of A and B demonstrates co-localization of TRPV1 and mGluR5 in DRG cells. Nuclei were stained using DAPI as the counterstain (blue).

 

Guinea Pig Polyclonals

Antibodies raised in guinea pig can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IH and IC.

Guinea pig Anti-TRPC6 Antibody (#AGP-002)

Guinea pig Anti-TRPV1 (VR1) Antibody (#AGP-118)

Guinea pig Anti-TRPV2 (extracellular) Antibody (#AGP-033)

 

Guinea pig Anti-TRPC6 Antibody (#AGP-002)

 

Expression of TRPC6 in rat cerebellum:

AGP-002

Immunohistochemical staining of rat cerebellum using Guinea pig Anti-TRPC6 Antibody (#AGP-002). A. TRPC6 (green) appears in the soma of Purkinje cells (white arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates intense expression of TRPC6 in the soma of Purkinje cells and lower expression in the molecular (Mol) layer. DAPI is used as the counterstain (blue).

Guinea pig Anti-TRPV2 (extracellular) Antibody (#AGP-033)

 

Immuno-colocalization of SERCA1 and TRPV2 in mouse heart:

AGP-033

Immunohistochemical staining of mouse heart immersion-fixed, free floating frozen sections, using rabbit Anti-SERCA1 Antibody (#ACP-011) (1:200) and Guinea pig Anti-TRPV2 (extracellular) Antibody (#AGP-033), (1:200). A. SERCA1 staining (green) appears in T tubules (arrows). B. TRPV2 staining (red) in same section is detected in intercalated discs (diagonal arrows) and T tubules (horizontal arrow). C. Merge of panels A and B demonstrates co-localization of SERCA1 and TRPV2. Nuclei are stained using DAPI (blue).

 

 

 

 

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