BioVision-Actin(g) on it: Actin Polymerization/Depolymerization Assay Kit

BioVision-Actin(g) on it: Actin Polymerization/Depolymerization Assay Kit

2018.03.31

 

Actin(g) on it: Actin Polymerization/Depolymerization Assay Kit

Hello Customer,

Actin (G- and F-actin) is often referred as the family of fibrillary proteins that can be found in almost all eukaryotes. In general, the formation of filamentous F-actin occurs when an Actin-ATP undergoes hydrolysis releasing inorganic phosphate (Pi) and ADP-Actin. F-actin is then formed by reversible, non-covalent processes using ADP-Actin molecules. Actin plays critical roles in cell mobility and cell contraction during cell division among others processes. BioVision is excited to introduce Actin Polymerization/Depolymerization Assay Kit. The assay utilizes proprietary pyrene-labeled actin that generates a stable signal if actin undergoes polymerization. Our assay is simple, high-throughput compatible, and can be used to study the effect of diverse potential anti-polymerization agents.

Key Features:    

  •  Non-radioactive, versatile assays (Microplate)
  • Specific, homogeneous assay
  • Sensitive: fluorometric format
  • Convenient: minimal sample preparation, fast protocols (< 3 hours)
  • Cost effective: 100 assays; High Throughput Screening (HTS) compatible
  • Adaptable: works with most of the commonly available instrumentations

     

Figures. A. Actin Polymerizarion using K457: Actin Polymerization is chemically induced (red). Actin polymerization is inhibited by Latrunculin A* (green). B. Actin Depolymerization: Polymerized Actin (red) is depolymerized by Latrunculin A (green). Assays were performed following the kit protocols. *Note: Latrunculin A is Actin polymerization inhibitor in vitro and in vivo by the formation of a 1:1 complex with monomeric G-actin. Latrunculin A acts a depolymerization agent acting on Actin filaments (F-actin).

Explore our diverse set of assay kits for the measurement of actin polymerization ATP, ADP, phosphate!:

Product Name

Cat.No. 

Size

Actin Polymerization/ Depolymerization Assay Kit (Fluorometric)

K457

100 assays

ADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR

K255

200 assays

ADP Colorimetric Assay Kit II

K356

100 assays

ADP Colorimetric/Fluorometric Assay Kit

K355

100 assays

ApoSENSORTM ATP Cell Viability Bioluminescence Assay Kit

K254

200, 1000 assays

ATP Colorimetric/Fluorometric Assay Kit

K354

100 assays

Bioluminescence Cytotoxicity Assay Kit

K312

500 assays

Cell Proliferation Assay Kit (Fluorometric)

K307

1000 assays

EZClickTM EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence

K946

100 assays

EZScreenTM ATP Colorimetric Assay Kit (384-well)

K959

400 assays

EZViableTM Calcein AM Cell Viability Assay Kit (Fluorometric)

K305

1000 assays

MTT Cell Proliferation Assay Kit (Colorimetric)

K299

1000 assays

PicoProbeTM ADP Assay Kit (Fluorometric)

K211

100 assays

PicoProbeTM Phosphate Fluorometric Assay Kit

K419

100 assays

Phosphate Assay Kit (Fluorometric)

K420

100 assays

Phosphate Colorimetric Assay Kit

K410

500 assays

Pyrophosphate (PPi) Assay Kit(Fluorometric/Colorimetric)

K568

100 assays

Quick Cell Proliferation Colorimetric Assay Kit

K301

500, 2500 assays

Quick Cell Proliferation colorimetric Assay Kit Plus

K302

500, 2500 assays

Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1

K304

2500 assays

StayBriteTM Highly Stable ATP Bioluminescence Assay Kit

K791

100, 1000 assays

 

 

 

Product Name

Cat.No. 

Size

Actin, pyrene labeled, Rabbit Muscle

M1319

500 μg, 1 mg

Actin, Rabbit Muscle

P1246

500 μg, 1 mg

Anti-Beta Actin Antibody (4F7)

A1031

100 µg

Curcubitacin E

B1592

1 mg, 5 mg

Cytochalasin D

B1176

1 mg, 5 mg

Jasplakinolide, jaspis johnstoni

1689

50 µg

Latrunculin A

2181

100 µg

Latrunculin B

2182

1 mg

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