BioVision-Are you sure your Antibodies are Endotoxin Free: Try BioVision’s Endotoxin Free Resins

BioVision-Are you sure your Antibodies are Endotoxin Free: Try BioVision’s Endotoxin Free Resins

2018.02.13

 Interested in Endotoxin-Free Antibody Purification?

Try BioVision’s Endotoxin-Free Resins

BioVision offers a wide range of Agarose and Sepharose coupled to Protein A and Protein G to purify monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. We are pleased to introduce our endotoxin-free resins (Protein A and G Agarose/Sepharose) for highly effective endotoxin free IgG purification and immunoprecipitation.The endotoxin-free Protein A and G resins are engineered using our proprietary technology consisting of purified recombinant protein A & G respectively that have been covalently coupled to 6% cross-linked Agarose/Sepharose beads. Our endotoxin-free resins are optimized for high IgG binding capacity, enhanced specificity, reusability and purification of endotoxin-free (< 0.1 EU/mg) IgG from serum or ascites samples.

Key Features:

·        Enhanced Specificity

·        Endotoxin-free: <1EU/mg

·        High Binding Capacity and High Flow Rate: >15 mg human or rabbit IgG/ml of settled resin

·        Minimal leaching of the ligand

·        Versatility: Binds a broad range of IgG species and subclasses

·        Reusable

·        Convenience: Available in multiple sizes

Figure. A. IgG purification with protein A-Sepharose (Endotoxin-Free) (Cat. No. M1300): IgG (10 mg) spiked with endotoxin (96 EU) is loaded onto 1 ml Endotoxin-free protein A-Sepharose. After purification, the recovered IgG shows significantly reduced endotoxin levels (More than 90% reduction).

Product Name

Cat. No.

Size

Protein A Sepharose (Endotoxin-free)

M1300

1 ml, 5 ml, 25 ml

Protein G Sepharose (Endotoxin-free)

M1301

1 ml, 5 ml, 25 ml

Protein A Agarose (Endotoxin-free)

M1320

1 ml, 5 ml, 25 ml

 

Check out BioVision’s other protein A/G/L resins:

 

Product Name

Cat. No.

Size

Hi-BindTM Protein A-Agarose

6520

1 ml, 5 ml, 25 ml, 100 ml, 500 ml

Hi-BindTM Protein G-Agarose

6513

1 ml, 5 ml, 25 ml, 100 ml

Hi-BindTM Cibacron Blue-Agarose Beads

7923

10 ml, 50 ml

Protein A-Agarose

6526

1 ml, 5 ml, 25 ml, 100 ml

Protein A-Sepharose

6501

1 ml, 5 ml, 25 ml, 100 ml

Protein G-Sepharose

6511

1 ml, 5 ml, 25 ml, 100 ml

Protein G Sepharose Column

6518

1 ml, 5 ml

Protein L-Sepharose

6531

1 ml, 5 ml, 25 ml, 100 ml

Protein L Sepharose Column

6538

1 ml, 5 ml

Protein A/G-Sepharose

6503

1 ml, 5 ml, 25 ml, 100 ml

Protein A/G-Sepharose Column

6528

1 ml, 5 ml

Protein A/G/L-Sepharose

6541

1 ml, 5 ml, 25 ml, 100 ml

Protein A/G/L-Sepharose Column

6548

1 ml, 5 ml

Protein G Magnetic Beads

6517

1 ml

Protein A/G/L Magnetic Beads

6547

1 ml

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