Having Problems Isolating Monocytes? You’re Not Alone…..
Monocytes are a type of white blood cells (leukocyte) and the largest of the immune cells. Monocytes, if correctly identified, are characterized by a large amoeboid shape, unilobar nucleus and vacuolated cytoplasm. However, monocytes are very difficult to identify in peripheral blood or bone marrow samples. Monocytes serve several roles: phagocytosis, presentation of antigen to T cells, and cytokine production. BioVision’s human Whole Blood Monocyte Isolation Kit allows for in vitro isolation of intact, viable monocytes. The kit enables the isolation of approximately 80% of total monocyte exhibiting sample purity greater than 95%. Additionally, we offer similar kits for the isolation of PBMC and PMNs.
· High Yield, viable PBMC and PMN cells (≥ 5 x106 cells per ml of human whole blood)
· Specific protocol for each cell type
· Convenient: minimal sample preparation; fast protocols
· Cost effective: 30 ml blood
· Validated: human blood
Figures. A. Isolation of Monocytes using K933. Layers of Density Barrier Solution and whole blood prior to and after centrifugation showing the separation of layers in the conical tube. B. Separation of four layers (monocytes, DBS, plasma, and RBCs). Legend: DBS: Density Barrier Solution, RBC: Red blood Cell.