BioVision-Having problems isolating Monocytes? You’re not alone…..

BioVision-Having problems isolating Monocytes? You’re not alone…..

2018.02.13

 

Having Problems Isolating Monocytes? You’re Not Alone…..

Hello Customer,

Monocytes are a type of white blood cells (leukocyte) and the largest of the immune cells. Monocytes, if correctly identified, are characterized by a large amoeboid shape, unilobar nucleus and vacuolated cytoplasm. However, monocytes are very difficult to identify in peripheral blood or bone marrow samples. Monocytes serve several roles: phagocytosis, presentation of antigen to T cells, and cytokine production. BioVision’s human Whole Blood Monocyte Isolation Kit allows for in vitro isolation of intact, viable monocytes. The kit enables the isolation of approximately 80% of total monocyte exhibiting sample purity greater than 95%. Additionally, we offer similar kits for the isolation of PBMC and PMNs.

Key Features (K933; Monocytes, K541; PMBC and K483; PMN isolation kits):    

·        High Yield, viable PBMC and PMN cells (≥ 5 x106 cells per ml of human whole blood)

·        Specific protocol for each cell type

·        Convenient: minimal sample preparation; fast protocols

·        Cost effective: 30 ml blood

·        Validated: human blood

     

Figures. A. Isolation of Monocytes using K933. Layers of Density Barrier Solution and whole blood prior to and after centrifugation showing the separation of layers in the conical tube. B. Separation of four layers (monocytes, DBS, plasma, and RBCs). Legend: DBS: Density Barrier Solution, RBC: Red blood Cell.

Cat. No.

Target Cell

Sample

Volume (ml)

Yield (cells/ml)

Purity (%)

K933

Monocytes

Human

Whole

Blood

30

5 X 106

> 95%

K483

Polymorphonuclear

30

5 X 106

K541

Peripheral Blood Mononuclear Cell

30

2.5 X 106


Additionally, explore our exclusive selection of hematopoietic cell differentiation kits:

TEL:+886-2-88663366   FAX:+886-2-28331789   MAIL:info@abscience.com.tw

11163台北市士林區文林路661巷1號1樓   1F., No.1, Ln. 661, Wenlin Rd., Shilin Dist., Taipei City 11163, Taiwan (R.O.C.)

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Cat.  #

Colony Types Detected

IL3

SCF

GM-CSF

EPO

TPO

G-CSF

IL6

Flt3-L

IL2

K3101

Primitive, expanding lympho-hematopoietic stem cell, B, E, G & M, Mk

+

+

+

+

+

+

+

+

K3102

GEM1,2,3,BFU-E, CFU-E, B, E, G & M, Mk, B-lymphocyte progenitor

+

+

+

+

+

+

+

K3103

GEM1,2,3, B,E,G&M, G, Mk

+

+

+

+

+

+

+

K3104

B,E,G&M, G

+

+

+

+

+

K3105

BFU-E, CFU-E

+

+

+

K3106

G&M

+

+

+

K3107

G&M, G, M