BioVision-(In)soluble collagen? Not anymore. Introducing Soluble Collagen Assay Kit

BioVision-(In)soluble collagen? Not anymore. Introducing Soluble Collagen Assay Kit

2018.03.31

 

Hello Customer,

Collagen is the most abundant protein in mammals, with more than twenty different characterized types. Types I, II and III comprise more than 90% of the total collagen in mammals. Collagen consists of three ‘preprocollagen’ strands with a repeating Glycine-X-Hydroxyproline sequence. While mature collagen is insoluble, tropocollagen is soluble in dilute acidic solutions. Tumor invasion, metastasis, and rheumatoid arthritis affect collagen synthesis, fibrillization and turnover. BioVision’s Soluble Collagen Assay Kit allows for quantification of newly synthesized (acid-soluble) collagen levels biological samples. The assay is simple, selective and more sensitive than other colorimetric-based methods..

·        Non-radioactive, homogeneous assays

·        Specific, sensitive assays

·        Convenient: minimal sample preparation; fast protocols

·        Cost effective: 100 assays; High Throughput Screening compatible

·        Validated: using mammalian tissues, biological fluids

The simplest, yet sensitive series of assays in the market!!!!

 

Fluorometric

Classic Method

PCA-Free Method

Detection

RFU (376/468 nm)

OD (560 nm)

OD (560 nm)

Hydroxyproline

N/A

K555

K226

Total Collagen

N/A

K218

K406

Soluble Collagen

K532

N/A

N/A

 

 

Hydroxyproline

Total Collagen

Soluble Collagen

 

K555

K226

K216

K406

K532

Range (µg)

0.1-1

0.1-1

0.5-10

0.5-10

0.2 – 2

 

 

Fluorometric

(Classic Method)

(PCA-Free)

Perchloric Acid

Not applicable

Yes

No

Hydrolysis (pH)

Acidic (Acetic Acid)

Acid (HCl)

Alkaline (NaOH)

Hydrolysis time (h)

8

3

1

Reaction Temperature (ºC)

37

60

60

Reaction time (min)

80

90

45

 

Figure (A). Estimation of acid-soluble collagen content in rat tissues. Rat tissue samples were homogenized in 0.5 M acetic acid, incubated overnight at 4°C to solubilize collagen and neutralized with 0.5 M NaOH. (B). Estimation of 3T3-L1 secreted soluble collagen in cultured cell growth medium (DMEM/F12 medium). Cells were treated with either vehicle or TGF-β (Cat. # 6479, a cytokine known to stimulate synthesis and secretion of tropocollagen fibrils) for 2 days. Media was assayed directly. TGF-β treatment resulted in a roughly 1.5-fold increase in collagen secretion.

Check out BioVision’s other Collagen and Amino Acid-Related Assay Kits!

 

Cat. No.

Product Name

Size

K490

Collagenase (Collagen egradation/Zymography) Assay Kit (Fluorometric)

100 Assays 

K792

Collagenase Activity Colorimetric Assay Kit

100 Assays

K833

Collagenase Inhibitor Screening Kit (Fluorometric)

100 Assays

K444

Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)

100 Assays

K531

Homocysteine Assay Kit (Fluorometric)

100 Assays

K652

Alanine Colorimetric/Fluorometric Assay Kit

100 Assays

K552

Aspartate Colorimetric/Fluorometric Assay Kit

100 Assays

K564

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

100 Assays

K558

Cysteine Assay Kit (Fluorometric)

100 Assays

K629

Glutamate Colorimetric Assay Kit

100 Assays

K556

Glutamine Colorimetric Assay Kit

100 Assays

K589

Glycine Assay Kit (Fluorometric)

100 Assays

K506

TEL:+886-2-88663366   FAX:+886-2-28331789   MAIL:info@abscience.com.tw

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