BioVision_Fall for these: One Click at a time!!!!

BioVision_Fall for these: One Click at a time!!!!

2017.10.11

 

 

                                          

 

 

 

 

 

 

When Chemistry and Biology meet: Instant Click!!!

Hello Customer,

 

Click Chemistry Reactions have been greatly utilized in Life Sciences and Pharmaceutical industries due to their unique advantages: high yield reactions with less undesired byproducts, maintain stereospecificity, fast and milder reaction conditions. Exciting, and novel applications include accurate, yet rapid measurement of biological processes such as cell viability, and de novo synthesis of RNA and DNA oligonucleotides. BioVision is proud to introduce EZClickTM Assay Kits, a series of products that could be used for the detection of biomolecules (DNA, RNA, Protein and Phospholipids).

 

As an appreciation of your business, and for a limited time, we are offering you a $25 US Amazon Gift Card with the purchase of any of our EZClickTM Assay Kits.*


Explore our products and allow us to accelerate your research: One Click at a time!

 

Key Features:

·        Non-radioactive, versatile assays (Flow cytometry and Fluorescence Microscopy)

·        Specific, homogeneous assay

·        Sensitive: fluorometric format

·        Convenient: minimal sample preparation, fast protocols (< 2 hours)

·        Cost effective: 100 assays; High Throughput Screening (HTS) compatible

·        Adaptable: works with most of the commonly available instrumentations

 

Cat. No.

Target

Biological Process

Label

Principle

Classical Assay

K946

DNA

Cell Cycle (S-Phase)

EdU

Replaces endogenous Thymidine into nascent DNA

BrdU

K718

RNA

RNA Transcription

5’-EU

Replaces endogenous Uridine into nascent RNA

BrU

K715

Protein

Protein Expression

OP-Puro

Stops protein translation via covalent bond with nascent polypeptides

Radioisotopes

K717

Phospholipid

Phospholipid Synthesis

Choline analog

Incorporation of Choline-based label into PC structure

HPLC, Enzymatic Reaction

 

Figure. A. Analysis of protein biosynthesis in presence of Cycloheximide. Jurkat were pre-incubated (vehicle vs. 1X Cycloheximide; 30 minutes at 37°C). Cells were then incubated either with culture medium (white), 1X EZClickTM Protein Label (Red) or 1X Cycloheximide (Green) for 30 minutes. Protein synthesis was detected by Flow Cytometry (FL-2). Data shows the inhibitory effect of Cycloheximide on nascent polypeptides synthesis. B. Analysis of protein biosynthesis in presence of Cycloheximide. HeLa cells were pre-incubated (vehicle vs. 1X Cycloheximide; 30 minutes at 37°C). Cells were then incubated in culture medium containing 1X EZClickTM Protein Label without or with 1X Cycloheximide. Protein synthesis was detected by Fluorescence Microscopy. Reduced red fluorescence in Cycloheximide shows its inhibitory effect on translation of mRNA to nascent polypeptides. Nuclear staining in both panels confirms that red fluorescence is the result of EZClickTM Protein Label incorporation.

 

*Terms and Conditions:


1. One (1) Amazon Gift Card (worth $25 US) will be sent for every EZClickTM kit that will be ordered between October 5th, and December 31st, 2017.
2. Promotion can be used multiple times during this period.
3. K946, K718, K715, K717 are the only products that are included in this offer.
4. Offer is only available for US customers with local address.
5. This promotional offer cannot be used in conjuction with any other promotion.

6. Use promo code: BV1017GC25

 

Check out BioVision’s other kits evaluating cell proliferation, cell cytotoxicity, cell viability and phospholipids content!

 

Product Name

Cat.No.

Size

Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)

K350

100 Assays

BrdU Cell Proliferation Assay Kit

K306

200, 1000 Assays

Cardiolipin Assay Kit (Fluorometric)

K944

100 Assays

Cell Proliferation Assay Kit (Fluorometric)

K307

1000 Assays

Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)

K315

100 Assays

Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit

K615

100 Assays

EZClick™ EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence

K946

100 Assays

EZClick™ Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K717

100 Assays

EZClick™ Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K715

100 Assays

EZViable™ Calcein AM Cell Viability Assay Kit (Fluorometric)

K305

1000 Assays

LDH-Cytotoxicity Colorimetric Assay Kit

K311

400 Assays

LDH-Cytotoxicity Colorimetric Assay Kit II

K313

500 Assays

Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

TEL:+886-2-88663366   FAX:+886-2-28331789   MAIL:info@abscience.com.tw

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