BioVision_Introducing BioVision’s New CRISPR Kits and Cas9 Proteins!

BioVision_Introducing BioVision’s New CRISPR Kits and Cas9 Proteins!

2017.10.11

 

Introducing BioVision’s New CRISPR Kits and Cas9 Proteins!

Hello Customer,

 

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 (CRISPR associated protein 9) system is the latest RNA-guided, endonuclease tool in genome editing which allows very specific genomic disruption and replacement. Cas9 protein of the CRISPR-Cas9 system is an RNA-guided DNA endonuclease enzyme that forms a very stable ribonucleoprotein (RNP) complex with the single-guide RNA (sgRNA) and unwinds the genomic DNA duplex. This leads to a disruption in the open reading frame of the targeted gene. BioVision offers a wide range of CRISPR tools including the highly pure recombinant S. pyogenes and S. aureus Cas9 Proteins, CRISPR Genomic Cleavage Detection Kit, and sgRNA Synthesis Kits for Genome Editing.

 

Applications:

·        RNA-guided Genome Editing

  • Gene Regulation

·        CRISPR Genomic Cleavage Detection Kit to Verify the Genomic Editing Process

·        CRISPR sgRNA Synthesis Kit for the Production of sgRNA

Key Features:

·        Ready-To-Use, Highly Pure and Efficient S. pyogenes and S. aureus Cas9 Proteins

·        Wide Range of Cas9 Proteins (Cas9 Nucleases, Nickases, Double Mutants) to Choose From

·        Injection and Transfection Ready Cas9 Proteins

·        GFP-Cas9 Proteins for Genomic Locus Visualization

·        Ready-to-Use, Easy & Effective CRISPR Genomic Cleavage Detection Kit to Verify Genomic Editing Process

·        Simple and Robust Production of sgRNA using CRISPR sgRNA Synthesis Kits 

In vitro cleavage assay comparison using Cas9 Nuclease NLS Protein. Lane 1: DNA+Cas9 (no sgRNA); Lane 2: DNA+Cas9 (sgRNA, 30 min); Lane 3: DNA+Competitor’s Cas9+sgRNA (30 min); Lane 4: DNA+Cas9+sgRNA (1hr); Lane 5: DNA+Competitor’s Cas9+sgRNA (1 hr).

 

Check out the full list of CRISPR/Cas9 products that BioVision has to offer:

 

Product Name

Cat. No.

Size

Classic CRISPR sgRNA Synthesis Kit

K1252

25 Reactions

CRISPR Genomic Cleavage Detection Kit

K1250

25 Reactions

Express CRISPR sgRNA Synthesis Kit

K1253

25 Reactions

Gene SnipperTM Cas9 GFP NLS

M1284

50 pmol, 250 pmol

Gene SnipperTM Cas9 GFP Null NLS

M1286

50 pmol, 250 pmol

Gene SnipperTM Cas9 Nickase GFP NLS

M1285

50 pmol, 250 pmol

GeneSnipper™ Cas9 Null

M1100

50 pmol, 250 pmol

GeneSnipper™ Cas9 Null NLS

M1103

50 pmol, 250 pmol

GeneSnipper™ Cas9 Protein

M1094

50 pmol, 250 pmol

GeneSnipper™ Cas9 NLS

M1095

50 pmol, 250 pmol

GeneSnipper™ Cas9 Nickase (D10A)

M1096

50 pmol, 250 pmol

GeneSnipper™ Cas9 (D10A) NLS

M1097

50 pmol, 250 pmol

GeneSnipper™ Cas9 Nickase (H840A)

M1098

50 pmol, 250 pmol

GeneSnipper™ Cas9 (H840A) NLS

M1099

50 pmol, 250 pmol

Gene SnipperTM SaCas9 Null

M1282

50 pmol, 250 pmol

Gene SnipperTM SaCas9 Null NLS

M1283

50 pmol, 250 pmol

Gene SnipperTM SaCas9 NLS

M1281

50 pmol, 250 pmol

Gene SnipperTM SaCas9 Protein

M1280

50 pmol, 250 pmol

 

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