BioVision_Phosholipids: Simple molecules, complex functions

BioVision_Phosholipids: Simple molecules, complex functions

2017.08.25

 

Phosholipids: Simple molecules, complex functions

Phospholipids are major components of the bilayers of all plasma membranes. Cho-containing phospholipids (Phosphatidylcholines; PC) are critical for structural membrane integrity, cellular metabolism and signaling, either as individual molecules or precursors of secondary messengers. Changes in synthesis of PCs are an essential parameter in analysis of cellular response to both, physiological and pathological conditions, environmental stress, or drug treatment. 

BioVision is proud to offer three versatile products for the quantification and detection of molecules in tissue lysates, or intact mammalian cells. EZClick™ Global Phospholipid Synthesis Assay Kit offers a method to visualize newly synthesized phospholipids in vivo using a click chemistry principle. Phospholipid Assay Kit and Phosphatidylcholne Assay Kit are accurate products that are able to measure total choline-containing phospholipids in samples such as serum, plasma and exosomes. These kits enable analyses of global biosynthesis, subcellular localization turnover of phospholipids and quantification of these molecules in cells/tissues/biofluids.

Key Features:

·        Non-radioactive, versatile assays (Flow cytometry and Fluorescence Microscopy)

·        Specific, homogeneous assay

·        Sensitive: fluorometric format

·        Convenient: minimal sample preparation, fast protocols (< 2 hours)

·        Cost effective: 100 assays; High Throughput Screening (HTS) compatible

·        Adaptable: works with most of the commonly available instrumentations

Figure. A. Determination of Phospholipid Concentration in Human Serum and plasma using K351-100. Normal concentrations in human ranges between 1 and 4 mM. (B) Analysis of metabolic labeling of phospholipids in proliferating cells using K717-100. Jurkat cells (1X106 cells/ml) were pre-treated with vehicle (black line) or cultured in presence of 1X EZClickTM Phospholipid Label (red line) for 24 hours at 37°C prior to 1 hour incubation with Phospholipase D (blue line). Modified phospholipid molecules were detected according to the kit protocol and red fluorescence was analyzed by FACS in FL-2 channel. Decrease in signal caused by hydrolysis of Cho-containing head groups via Phospholipase D activity confirms that red fluorescence is the result of EZClickTM Phospholipid Label incorporation.

 

Check out BioVision’s other kits evaluating lipid metabolism:

 

 

Product Name

Cat.No.

Size

ADP Colorimetric Assay Kit II

K356

100 assays

Acetoacetate Colorimetric Assay Kit

K650

100 assays

β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit

K632

 100 assays

PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit

K651

100 assays

Total Bile Acids (TBA) Assay Kit (Colorimetric)

K209

100 assays

Cardiolipin Assay Kit (Fluorometric)

K944

100 assays

Cholesterol Detection Kit (cell-based)

K587

100 assays

Cholesterol Efflux Fluorometric Assay Kit (cell-based)

K582

100 assays

EZScreen™ Total Cholesterol & Cholesteryl Ester Colorimetric Assay Kit (384-Well)

K957

400 assays

Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit

K603

100 assays

Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II

K623

100 assays

Free Fatty Acid Quantification Colorimetric/Fluorometric Kit

K612

100 assays

EZScreen™ Beta-Lactamase Activity Colorimetric Assay Kit (384-well)

K954

400 assays

Free Glycerol Colorimetric/Fluorometric Assay Kit

K630

100 assays

PicoProbe™ Free Glycerol Fluorometric Assay Kit

K643

100 assays

Glycerol Cell-Based Assay Kit

K977

100 assays

HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit

K613

100 assays

HDL Uptake Assay Kit (Fluorometric)

K586

100 assays

Sphingomyelin Quantification Colorimetric Assay Kit

K600

100 assays

Triglyceride Quantification Colorimetric/Fluorometric Kit

K622

100 assays

PicoProbe™ Triglyceride (TG) Fluorometric Assay Kit

K614

100 assays

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