BioVision_RNA:Good programmers need only 4 commands

BioVision_RNA:Good programmers need only 4 commands

2017.08.25

 

Cells: The first programmers

RNA plays crucial role in coding, decoding and regulation of genes, and protein expression in all living cells. The ability to detect newly synthesized RNA or changes in RNA levels under various physiological conditions, or resulting from disease, environmental damage, or drug treatments is an important aspect of toxicological profiling. Many anti-cancer drugs inhibit transcription, and most transcription inhibitors have useful pharmacological properties. Biovision’s EZClick™ Global RNA Synthesis Assay Kit and EZClick™ EdU DNA Synthesis Monitoring Kit provide simple and robust tools for detection of global RNA and DNA respectively. Our kits can detect de novo synthesized RNA or DNA following simple procedures that are carried out under mild conditions and without the use of radiolabeling or antibodies using FACS or fluorescence microscopy.

Key Features:

·        Non-radioactive, versatile assays (Flow cytometry and Fluorescence Microscopy)

·        Specific, homogeneous assay

·        Sensitive: fluorometric format

·        Convenient: minimal sample preparation, fast protocols (< 2 hours)

·        Cost effective: 100 assays; High Throughput Screening (HTS) compatible

·        Adaptable: works with most of the commonly available instrumentations

Figure. A. Analysis of RNA biosynthesis in presence of Actinomycin using K718-100 D. (A) Jurkat cells (1X106 cells/ml) were pre-treated with vehicle (black line) or 1 X Actinomycin D (blue line) for 4 hours at 37°C prior to 1 hour incubation with EZClickTM RNA Label (red line). Cells were then processed for detection of de novo synthesized RNA according to the included protocol. Fluorescence measured in FL-2 channel clearly shows the inhibitory effect of Actinomycin D on RNA synthesis. (B) HeLa cells (105 cells/ ml) were pre-treated either with vehicle (top) or 1 Actinomycin D (bottom) for 4 hours at 37°C prior to 1 hour incubation with EZClickTM RNA Label. De novo synthesized RNA was detected by Fluorescence Microscope. Reduced red fluorescence in panel B confirms the inhibitory effect of Actinomycin D on RNA biosynthesis. Nuclear staining in both panels confirms that red fluorescence is the result of EZClickTM RNA Label incorporation.

 

Check out BioVision’s other kits evaluating cell proliferation, cell cytotoxicity and cell viability !:

 

Product Name

Cat.No.

Size

EZClick™ EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence

K946

100 assays

EZClick™ Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence

K718

100 assays

BrdU Cell Proliferation Assay Kit

K306

200 assays, 1000 assays

Cell Proliferation Assay Kit (Fluorometric)

K307

1000 assays

EZViable™ Calcein AM Cell Viability Assay Kit (Fluorometric)

K305

1000 assays

MTS Cell Proliferation Colorimetric Assay Kit

K300

500 assays, 2500 assays

MTT Cell Proliferation Assay Kit (Colorimetric)

K299

1000 assays

Neutral Red Cell Cytotoxicity Assay Kit

K447

1000 assays

Quick Cell Proliferation Colorimetric Assay Kit

K301

500 assays, 2500 assays

Quick Cell Proliferation colorimetric Assay Kit Plus

K302

500 assays, 2500 assays

Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1

K304

2500 assays

VisionBlue™ Quick Cell Viability Fluorometric Assay Kit

K303

500 assays, 2500 assays

Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)

K350

100 assays

Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)

K315

100 assays

LDH-Cytotoxicity Colorimetric Assay Kit

K311

400 assays

LDH-Cytotoxicity Colorimetric Assay Kit II

K313

500 assays

PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit

K314

500 assays

Live-Dead Cell Staining Kit

K501

100 stainings

Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

K502

100 assays

Senescence Detection Kit

K320

250 Stainings

Senescence Detection Kit (Fluorometric)

K991

100 assays

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