BioVision´s Glycoprotein Assay Kits

BioVision´s Glycoprotein Assay Kits

2018.06.01

 

 
 
Hello Customer,
Glycans are vital components of glycoproteins, glycolipids, and proteoglycans. Glycosylation occurs co- or post-translationally on eukaryotic resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Since glycoproteins are not directly encoded in the genome, methods of their characterization and analyses are of great interest. BioVision offers four exciting Assay Kits that enable the measurement of glyco-modifications. We utilize glycan-analogs that are fed directly into the cells, processed via metabolic pathways and incorporated into the glycoproteins. These assay kits utilize our proprietary Click Chemistry technology, offering powerful, specific methods for imaging the localization, trafficking and dynamics of glycans, or detection. Take advantage of our ongoing promotion of 10% off on metabolic labeling kits
Key Features:
  • Non-radioactive, versatile assays (Flow cytometry and Fluorescence Microscopy)
  • Specific, homogeneous assay
  • Sensitive: fluorometric format
  • Convenient: minimal sample preparation, fast protocols (< 2 hours)
  • Cost effective: 100 assays; High Throughput Screening (HTS) compatible
  • Adaptable: works with most of the commonly available instrumentations
 
Glycosylation
Covalent bond
Glycoprotein-Modified Amino Acid
NGlycan
Side Chain Nitrogen of an AA
Asparagine
Arginine
OGlycan
Side Chain Oxygen of an AA
Serine (K743-100)
Threonine (K463-100)
Tyrosine (K573-100)
Hydroxyproline (K555-100; K226-100)
Phosphoglycan
Phosphate Group
Phosphoserine
CGlycan
Side Chain Carbon of an AA
Tryptophan (K557-100)
 
Target
NANA
GalNAc
GlcNAc
Fucose
Click label
Ac4ManAz
Ac4GalAz
Ac4GlcNAz
FucAz
CL incubation (h)
24-72
Size
100 assays
Cells
Suspension/Adherent
Applications
Fluorescence Microscopy/Flow Cytometry
Ex/Em (FACS) nm
488/(530/590)
Ex/Em (FC) nm
(440/490)/(540/580)
Rxn Time
30 min
Fig. Fluorescence Microscope images of subcellular localization (middle panel) of Fucosylated glycoproteins in fixed HeLa cells. Nuclear staining (left panel) and composite image (right panel).
Check out BioVision’s other kits evaluating cell proliferation, cell cytotoxicity, cell viability and protein carbonyl content!
Product Name
Cat. No.
Size
Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)
100 assays
BrdU Cell Proliferation Assay Kit
200, 1000 assays
Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit
100 assays
Cardiolipin Assay Kit (Fluorometric)
100 assays
Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)
100 assays
Cell Proliferation Assay Kit (Fluorometric)
1000 assays
Deproteinizing Sample Preparation Kit
200 assays
Deproteinizing Sample Preparation Kit II
200 assays
EZClickTM Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
100 assays
EZClickTM EdU Cell Proliferation/DNA Synthesis Kit (FACS/Microscopy), Red Fluorescence
100 assays
EZClickTM Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence
100 assays
EZViableTM Calcein AM Cell Viability Assay Kit (Fluorometric)
1000 assays
LDH-Cytotoxicity Colorimetric Assay Kit II
500 assays
LDH-Cytotoxicity Colorimetric Assay Kit
400 assays
Live/Dead Cell Viability Assay Kit (for Mammalian Cells)
100 assays
Live-Dead Cell Staining Kit
100 stainings
MTT Cell Proliferation Assay Kit (Colorimetric)

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