Please welcome FabRICATOR®-HPLC to the SmartEnzymes™ family!
FabRICATOR-HPLC can be used in a standard LC-MS setup for routine analysis. But more advanced configurations are possible, for example with a 2D-LC. Ultimately a bioreactor can be connected directly to the MS in an automated online middle-level workflow. This would significantly reduce operator time, sample handling errors and increase throughput.
To demonstrate the potential, we added β1-4-Galactosidase (β-gal) to trastuzumab and determined the changes in the Fc glycan profile at different time points using middle-level analysis with FabRICATOR-HPLC. The resulting Fc glycan spectra show the transformation as mAb galactosylation decreased over a period of 18 hours.
FabRICATOR-HPLC is versatile and gives consistent subunit generation for three other well-known therapeutic antibodies, adalimumab, rituximab and cetuximab. All three are completely digested as seen by the lack intact HC detected in their UV chromatograms. Their respective Fc glycan profiles show glycosylation patterns in accordance to previously published results. The FabRICATOR-HPLC column can be applied to human IgG1-4, to fusion proteins, ADCs, bispecific antibodies and other molecules for which FabRICATOR is active.
• Automated subunit generation
• Digests 10 µg IgG in 5 minutes
• No carry-over observed in tests
• Increased sample throughput
• Reduced sample handling
Genovis attened the CASSS MS conference in San Fransisco last week to present a poster on automated glycan analysis using FabRICATOR-HPLC digestion. Get a copy of the poster here and read more about our new technology.