Basic Affinity-Purified Polyclonal Antibody Package 基本型親和性純化多克隆抗體代製(雞)

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SC1370

客製化

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Description

不同於單克隆抗體,多克隆抗體對應於多表位,往往包含若干免疫球蛋白亞型。它們可以用於Westerns,ELISAs and IHC。我們保證由GenScrip做肽抗原設計,合成及免疫的抗體在任何物種於ELISA將有稀釋比例1:20,000或更好。對於任何蛋白抗原(3毫 克, “純度80%,90%首選),我們保證了積極的抗體免疫印跡結果,並用ELISA稀釋比例1:20,000或更好。否則我們將免費重新啟動該項目免費, 直至保證滿意。我們的動物設施經過AAALAC認證的國際認可。

Includes

  • Conjugation to KLH (optional)
  • Two chickens, pre-immune bleed, four immunizations/chicken
  • Antibody purification by peptide affinity column from yolk
  • ELISA test
  • Lyophilization
  • If the antigen is provided by the customer, 2 mg of peptide or protein is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents. Another 5 mg of antigen is required for purification
Delivery

  • 1-2 ml of pre-immeune serum (lyophilized)*
  • 4 ml of antisera (lyophilized)
  • ELISA results
  • Affinity-purified IgY antibodies (lyophilized)
Time frame

  • Conjugation: 1 week
  • Immunization: 10-11 weeks
  • Purification and ELISA: 2 week
  • Lyophilization and Shipping: 1-2 weeks
  • Total 14-16 weeks

*收集和純化的抗體分離動物,共2毫升的免疫前血清(1毫升的各2隻)提供。對於抗體純化的混合血清,提共1毫升的免疫前血清。

備註

如果沒有具體要求,0.02% w/v sodium azide將被添加在抗血清或抗體產品,以防止細菌滋生。凍乾延長了抗體的壽命,消除了漏水問題,減輕了嚴格的運輸溫度要求,減少了抗體的總重量。提供所有的抗血清或純化抗體凍乾形式,除非特殊的要求.

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