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Basic Monoclonal Antibody Package (Mouse) 基本型單克隆抗體代製(å¤§é¼ )
SC1391
客製化
Brand
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Description
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Phase I: Immunization and ELISA screening. Time: 8-10 weeks.
- Conjugation: The peptide or small protein is conjugated to a carrier protein, such as KLH. Normally, 2 mg of protein immunogen or 5-10 mg of peptide is required.
- Immunization: 5 Balb/c mice (3 rats) are immunized and boosted with standard immunization protocol.
- Screening: Tail bleedings are screened by ELISA or other method if needed.
Phase II: Fusion and screening. Time: 4-8 weeks.
- Fusion: Up to 2 fusions (1-2 spleens per fusion) are performed on the best mice that responded the best to the immunization. Additional fusions are available at an extra charge.
- Screening: The hybridoma cells are expanded and screened by ELISA or other method if needed to obtain satisfied clones.
Phase III:Subcloning, expansion, cryopreservation and delivery. Time: 4-6 weeks.
- Subcloning: Subclone up to 5 parental clones by limiting dilution and isotyped, expand and freeze 2 subclones for each parental clone.
- Delivery: Two vials of cell culture for each subclone and 5 ml of culture supernatants from the clones is delivered to the customer. Additional parental clones are available at an extra charge.
Total Time: 4-6 months.
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Application
Reactivity