Biotinylated Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc,Avitag™
CD8-H82F2
200ug
Brand
ACROBiosystems
Description
Source :
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (CD8-H82F2) is expressed from human 293 cells (HEK293). It contains AA Asn 19 – Pro 152 (Accession # AAH93698). In the region Asn 19 – Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
Molecule : CD28
Synonyms : CD28,Tp44
Format : Powder
Category : MABSol® Biotin Labeled Proteins
Accession : AAH93698
Storage : -20℃
Shipping condition : Powder,RT
Molecular Weight : 43.6 kDa
Characteristics :
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level : Less than 1.0 EU per μg by the LAL method.
Buffer : Tris with Glycine, Arginine and NaCl, pH7.5
Description :
T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
References :
(1)Evans E.J., et al., 2005, Nat. Immunol. 6:271-279.
(2) Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46.
(3) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
Application
Reactivity