Biotinylated Mouse TNF-alpha Protein, His,Avitag™ (active trimer) (MALS verified)

Source : 

MABSol® Biotinylated Mouse TNF-alpha, His,Avitag (active trimer) (MALS verified) (TNA-M82E9) is expressed from human HEK293 cells. It contains AA Leu 80 – Leu 235 (Accession # P06804).

Molecule : TNF-alpha

Synonyms : DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

Format : Powder

Category : MABSol® Biotin Labeled Proteins

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 20.2 kDa

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 20.2 kDa. The protein migrates as 21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : PBS, pH7.4

Description :

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer’s disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

References :

(1) Dowlati Y, Herrmann N, et al., 2010, Biol Psychiatry 67 (5): 446–457.
(2) Swardfager W, Lanctot K, et al., 2010, Biol Psychiatry 68 (10): 930–941.
(3) Locksley RM, Killeen N et al., 2001, Cell 104 (4): 487–501.