Biotinylated Mouse VEGF164 Protein, His,Avitag™

Source : 

Biotinylated Mouse VEGF164, His,Avitag (VE4-M82Q3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 – Arg 190 (Accession # Q00731-2).

Molecule : VEGF164

Synonyms : RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

Format : Powder

Category : MABSol® Biotin Labeled Proteins

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 22.5 kDa

Characteristics :

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 22.5 kDa. As a result of different glycosylation, the protein migrates as 28-33 kDa under reducing (R) condition, and 46-60 kD

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : PBS, pH7.4

Description :

Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

References :

(1) Shin S.-Y., et al., 2005, Yonsei Med. J. 46:679-686.
(2) Breier G., et al., 1992, Development 114:521-532.