Cynomolgus / Rhesus macaque TIGIT Protein, Fc Tag

Source : 

Cynomolgus / Rhesus macaque TIGIT, Fc Tag (TIT-C5254) is expressed from human 293 cells (HEK293). It contains AA Met 89 – Ile 208 (Accession # G7NXM4-1). In the region Met 89 – Ile 208, the AA sequence of Cynomolgus and Rhesus macaque TIGIT are homologus.

Molecule : TIGIT

Synonyms : TIGIT,VSIG9,VSTM3

Format : Powder

Category : Immune Checkpoint Proteins

Accession : XP_015300911.1

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 39.9 kDa

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.9 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : 50 mM Tris, 100 mM Glycine, pH7.5

Description :

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

References :

(1) Yu X., et al., 2009, Nat. Immunol. 10:48-57.
(2) Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
(3) Lozano E., et al., 2012, J. Immunol. 188:3869-3875.