Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein (GP), His Tag

Source:

Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag (ZE5-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 – Asp 637 (Accession # AAQ55048.1).

Molecule : Glycoprotein / GP (virus)

Synonyms : GP,Envelope glycoprotein,GP(1,2)

Format : Powder

Category : Viral Proteins

Accession : AAQ55048.1

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 67.2 kDa

Characteristics :

Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67.2 kDa. The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : PBS, pH7.4

Description :

EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

References :

(1) Saeed M.F., et al., 2010, PLoS Pathog. 6:0-0.
(2) Marzi A., et al., 2006, J. Virol. 80:6305-6317.
(3) Chandran K., et al., 2005, Science 308:1643-1645.