ER-O™ Endoplasmic Reticulum Tracing Reagent

ZZZZ_Status (Active, Disc) : Discontinued

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

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(Antibody only) :

Application_Details :

Fluorescent imaging of the endoplasmic reticulum compartment.

Sample types: live cells, fixed cells (4% paraformaldehyde), tissue (frozen only)
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: NO
Raman spectroscopy: NO
Ex/Em: 405 nm / 570 nm

Typical working dilution is 1:100 – 1:200 (50-100 µM) in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Background_Info : TR-601-ER1 is a cell-permeant stain for the endoplasmic reticulum. TR-601-ER1 passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. It can be used as a real-time imaging reagent which can be imaged within minutes of addition and has minimal photobleaching. TR-601-ER1 is easily washed from cells, and therefore is ideal for protocols which require intermittent monitoring of endoplasmic reticulum structures. This tracing reagent can be used on live and fixed samples and is compatible with other dyes. TR-601-ER1 has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-601-ER1 is manufactured by ReZolve Scientific.

Clone_Name
(AB only, monoclonals) :

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=-1645

Description (Long Description) : 

TR-601-ER1 is a cell-permeant stain for the endoplasmic reticulum. TR-601-ER1 passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. It can be used as a real-time imaging reagent which can be imaged within minutes of addition and has minimal photobleaching. TR-601-ER1 is easily washed from cells, and therefore is ideal for protocols which require intermittent monitoring of endoplasmic reticulum structures. This tracing reagent can be used on live and fixed samples and is compatible with other dyes. TR-601-ER1 has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-601-ER1 is manufactured by ReZolve Scientific.

Expiry_Date : 

Lyophilised product is stable for 6 months at room temperature if protected from light. After reconstitution in DMSO, liquid 10 mM stock solution is stable for 6 months at room temperature.

Format (Form) : 

Dry, synthetic chemical powder

Immunogen (Antigen) :

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