Goat anti-OGT Antibody

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

OGT; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); FLJ23071; HRNT1; MGC22921; O-GLCNAC ; O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase

Antibody_Isotype
(Antibody only) :

Application_Details :

Western Blot: Approx 110kDa band observed in Rat Pancreas lysates calculated MW of 116kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.05-0.2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Peptide ELISA: antibody detection limit dilution 1:64000.

Background_Info :

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(AB only, monoclonals) :

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Description (Long Description) : 

Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1 and can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (Uniprot)..

Expiry_Date : 

12 months after purchase if unopened.

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-YEHPKDLKLSDGR

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