GST-UbcH2 (E2-14K)

PDF

E2-605

50 μg 100 μg

Brand

Boston Biochem

Description

GST-UbcH2 (E2-14K)

Catalog #: E2-605

GST-UbcH2 (E2-14K)
Product Source: 

human recombinant

This E2 enzyme encodes for the human homolog of the yeast Ubc8 gene. UbcH2 can conjugate ubiquitin to histone H2A in an E3-independent manner in vitro. This E2 enzyme is also involved in the ubiquitination of N-end rule pathway substrates, and can function in conjugation with the E3α ligase enzyme. Additionally, UbcH2 may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia. Accession # NP_00335. This protein has an N-terminal GST tag.

Product Information
Quantity: 

50 μg 100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific lot number.

MW: 

43 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

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