HCoV-229E S1 protein, His Tag

Source : 

HCoV-229E S1 protein, His Tag (SIN-V52H4) is expressed from human 293 cells (HEK293). It contains AA Cys 16 – Ala 536 (Accession # P15423-1).

Molecule : S1 protein

Synonyms : S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1,COVID-19

Format : Powder

Category : Viral Proteins

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 58.8 kDa

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : PBS, pH7.4

Description :

It’s been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

References :

(1) Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
(2) Benvenuto D, et al. J Med Virol. 2020.
(3) Chang CY, et al. AMB Express. 2020. 10(1):20.