His6-Apg3p

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E2-670

50 μg 100 μg

Brand

Boston Biochem

Description

His6-Apg3p

Catalog #: E2-670

His6-Apg3p
Product Source: 

human recombinant

Also known as: His6-Apg3p (Apg3L)

Apg3p is the E2-like enzyme necessary for the covalent conjugation of human Apg8 modifier proteins to lipid phosphatidylethanolamine (PE) and is essential in autophagosomal membrane formation. The Apg8 ubiquitin-like proteins (including GABARAP, GATE-16, MAP-LC3) are first activated by the E1-like activating enzyme Apg7p. Apg7p forms a complex with Apg3p which subsequently forms a glycyl thiolester bond with Apg8 proteins through its active site cysteine (C264). Accession # NP_071933.

Product Information
Quantity: 

50 μg 100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific lot number.

MW: 

36.8 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

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