His6-Hdm2 (mdm2)

PDF

E3-200

50 μg

Brand

Boston Biochem

Description

His6-Hdm2 (mdm2)

Catalog #: E3-200

His6-Hdm2 (mdm2)
Product Source: 

human recombinant

Hdm2 (Human double minute 2) is a RING-finger ubiquitin E3 ligase that acts as a major regulator of the tumor suppressor p53. Hdm2 inhibits p53-mediated transactivation which leads to deregulated cell proliferation. The E3 ligase activity is confined to the C-terminal domain of Hdm2 and is responsible for the ubiquitination and subsequent proteasomal degradation of p53. Although the isolated RING domain is capable of p53 ubiquitination, other regions of the protein including a central acidic domain are also crucial for full E3 ligase activity. Hdm2 also regulates its own intracellular levels by auto-ubiquitination, and can be SUMOylated which decreases auto-ubiquitination activity but increases activity toward p53. Hdm2 also affects the cell cycle and apoptosis through interactions with other proteins such as retinoblastoma1 (pRB) and ribosomal protein L5. This protein is full-length.

Product Information
Quantity: 

50 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies by lot number.

MW: 

60 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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