His6-MurF1

PDF

E3-100

25 μg

Brand

Boston Biochem

Description

His6-MurF1

Catalog #: E3-100

His6-MurF1
Product Source: 

human recombinant

MurF1 (Muscle-specific RING-finger protein 1) is a RING-finger E3 ligase found in striated muscle (heart and skeletal) and iris tissues. MurF1 shares 62% and 77% sequence homology with MurF2 and MurF3 respectively. The protein contains a RING-finger/B-box/coiled-coil tripartite fold known as TRIM, and it can form homo- and hetero-oligomers via the coiled-coil dimerization motifs. The N-terminal E3 ligase activity regulates the proteasomal degradation of cardiac troponin and probably other sarcomeric-associated proteins. The exact cellular roles and substrates for MurF1 are not yet known but it interacts with various proteins including titin, Isopeptidase3, SUMO-3, UbcH9 and GMEB-1. MurF1 may have a role in muscle adaptation, atrophy and hypertrophy mediated by ubiquitin-proteasome pathways (UPP). Muscle atrophy is associated with many diseases including cancer, diabetes and AIDS. This protein contains an N-terminal His6 tag.

Product Information
Quantity: 

25 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.

MW: 

40 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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