His6-UbcH10, Dominant Negative

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His6-UbcH10

100 μg

Brand

Boston Biochem

Description

His6-UbcH10, Dominant Negative

Catalog #: E2-652

His6-UbcH10, Dominant Negative
Product Source: 

human recombinant

UbcH10 is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex. This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH10 to transfer ubiquitin to an accepting E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions.

Product Information
Quantity: 

100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.

MW: 

20 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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