His6-Ube2E3

PDF

E2-678

50 μg 100 μg

Brand

Boston Biochem

Description

His6-Ube2E3

Catalog #: E2-678

His6-Ube2E3
Product Source: 

human recombinant

Ube2E3 is a Class III E2 protein with unique C- and N-terminal extension in addition to the conserved UBC domain. This enzyme shares over 90% identity with UbcH6, mouse UbcM2 and Drosophila UbcD2, but the protein N-termini are divergent. This E2 enzyme is active in conjugation reactions with various E3 ligase enzymes (BRCA1, CHIP), and is known to interact with two RING-Finger proteins, ARA54 and RNF8, via its UBC domain. This protein has an N-terminal His6-tag. (Accession # NP_006348.1)

Product Information
Quantity: 

50 μg 100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.

MW: 

24.9 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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