His6-Ube2F

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E2-679

50 μg 100 μg

Brand

Boston Biochem

Description

His6-Ube2F

Catalog #: E2-679

His6-Ube2F
Product Source: 

human recombinant

Ube2F accepts the ubiquitin-like protein NEDD8 from the NEDD8E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-Ube2F complex modifies specific target proteins, such as CUL5. UbcH12 (Ube2M) is another NEDD8 E2 conjugating enzyme which interacts with RBX1 and targets different types of cullin substrates. The Ube2F and Ube2M enzymes thus represent a hierarchical expansion of the NEDD8 conjugation system in establishing selective culling RING ligase activation which influences substrate sub-type NEDDylation. This protein has an N-terminal His6-tag. Accession # NP_542409.

Product Information
Quantity: 

50 μg 100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.2, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.

MW: 

23.2 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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