His6-Ube2G2

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E2-680

50 μg 100 μg

Brand

Boston Biochem

Description

His6-Ube2G2

Catalog #: E2-680

His6-Ube2G2
Product Source: 

human recombinant

Ube2G2 is a Class I E2 enzyme and shares 60% and 100% sequence identity with S.cerevisiae Ubc7 and mouse respectively. In yeast, Ubc7 is a endoplasmic reticulum (ER)-bound molecule and the active site faces the cystosol. The Ube2G2 E2 enzyme and the GP78 E3 ligase are active components of ERAD (endoplasmic reticulum-associated degradation) pathway which is essential for the degradation of misfolded ER proteins. The mechanism of K48-linked poly-ubiquitination by Ube2G2/GP78 appears to involve the transfer of preassembled Ub chains from Ube2G2 to lysine residues in a substrate. The E2 and E3 enzymes form a large hetero-oligomer which brings multiple Ube2G2 molecules into close proximity which allows for Ub transfer between neighboring E2s. This Ube2G2 contains an N-terminal His6 tag. Accession # NP_003334.

Product Information
Quantity: 

50 μg 100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.2, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.

MW: 

20.5 kD

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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