His6-USP7FL

PDF

E-519

25 μg

Brand

Boston Biochem

Description

His6-USP7FL

Catalog #: E-519

His6-USP7FL
Product Source: 

human recombinant

Also known as: His6-USP7FL (HAUSP)

USP7 (Herpes-Associated Ubiquitin Specific Protease) is a nuclear protein that was initially identified as a novel p53-interacting protein. The enzyme deubiquitinates p53 thus stabilizing the levels of this key tumor suppressor, and inducing p53-dependent cell growth repression and apoptosis. USP7 also indirectly affects p53 stability by deubiquitinating HDMX, a regulator of HDM2. Full-length USP7 (1102 amino acids) contains an N-terminal p53 binding domain and 2 highly conserved USP active site domains. The C-terminal domain interacts with HSV-1 protein ICP0. This enzyme is able to cleave model substrates including linear Ub fusions, poly- ubiquitinated EBNA1 and poly-ubiquitinated p53. This recombinant protein has an N-terminal his tag & residues 213-548 representing the catalytic domain (CD) of USP7. Accession # NP_003461.

Product Information
Quantity: 

25 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Concentration varies with Lot #.

MW: 

128 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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