His6-USP8

PDF

E-520

25 μg

Brand

Boston Biochem

Description

His6-USP8

Catalog #: E-520

His6-USP8
Product Source: 

human recombinant

Also known as: His6-USP8 (UBPY)

USP8 (Ubiquitin Specific Protease8) is a growth-regulated deubiquitinating enzyme (DUB) with a role in endosomal sorting of receptor tyrosine kinases (RTKs) such as EGFR in vivo USP8 is a complex protein with a coiled-coil region, non-classical SH3 binding motif, rhodanese and catalytic domains whose individual structures have been determined. The N-terminus has a novel dimerization fold but it is not known if the full-length protein exists as a homodimer. The C-terminus contains the DUB catalytic center and a nearby Zn++ ribbon subdomain which might have a role in poly-Ub binding specificity and substrate-induced conformational changes. USP8 has DUB activity on Ub chains in vitro and does not discriminate between K48- and K63-linked isopeptide bonds. USP8 interacts with several proteins including Hrs binding protein Hbp, Mona/Gads, Ras exchange factor CDC25Mm, GRAIL3 and NRDP1 E3 ligases, and 14-3-3ε. The overall function of USP8 is still unclear but its main role is in endocytosis which influences signaling cascades for mitogenesis, cell survival, differentiation and migration. This protein contains an N-terminal His6-tag.

Product Information
Quantity: 

25 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Concentration varies with Lot #.

MW: 

131 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

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