Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc Tag (HPLC-verified)

Source : 

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (CD8-H525a) is expressed from human 293 cells (HEK293). It contains AA Asn 19 – Pro 152 (Accession # P10747-1). In the region Asn 19 – Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.

Molecule : CD28

Synonyms : CD28,Tp44

Format : Powder

Category : Immune Checkpoint Proteins

Accession : NP_006130.1

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 41.6 kDa

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

Description :

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

References :

(1) Evans E.J., et al., 2005, Nat. Immunol. 6:271-279.
(2) Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46.
(3) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.