Human IL-6 / Interleukin-6 ELISA Pair Set
SEKB10395
Brand
Sino Biological
Description
分子 : IL6
保存條件:
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
線性範圍 : 9.38-600
包被抗體 : 1 mg/mL of mouse anti-Human IL-6 / Interleukin-6 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10395-MM14)
檢測抗體: 0.2 mg/mL of mouse anti-Human IL-6 / Interleukin-6 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.1 μg/mL in Dilution Buffer before use. (Catalog: # 10395-MM72)
標準品 : Each vial contains 35 ng of recombinant Human IL-6 / Interleukin-6. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 600 pg/mL is recommended.
生物素交聯HRP:
靈敏度 : The minimum detectable dose of Human IL-6 / Interleukin-6 was determined to be approximately 9.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
特異性 :
檢測原理 : The Human IL-6 / Interleukin-6 ELISA Pair Set is for the quantitative determination of Human IL-6 / Interleukin-6.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human IL-6 / Interleukin-6 coated on a 96-well plate. Standards and samples are added to the wells, and any Human IL-6 / Interleukin-6 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human IL-6 / Interleukin-6 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human IL-6 / Interleukin-6 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
檢測限: 9.38 pg/ml
血清樣本檢測範圍 :
Application
Reactivity