Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein, His Tag&Tag Free

Source : 

Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-H52W4) is expressed from human 293 cells (HEK293). It contains AA Phe 17 – Asn 1105 (ITGAM) & Gln 23 – Asn 700 (ITGB2) (Accession # P11215-2 (ITGAM) & P05107-1 (ITGB2)).

Molecule : Integrin alpha M beta 2

Synonyms : Integrin alpha M beta 2,ITGAM&ITGB2

Format : Powder

Category : Bio-Markers & CD Antigens

Accession : NP_001139280.1 (ITGAM) & NP_000202.3 (ITGB2)

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 127.2 kDa (ITGAM) & 80.2 kDa (ITGB2)

Characteristics :

Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAM and ITGB2, has a calculated MW of 127.2 kDa (ITGAM) and 80.2 kDa (ITGB2). Subunit ITGAM is fused with an acidic tail at the C-terminus and followed by a polyhistid

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : 50 mM Tris, 150 mM NaCl, pH7.5

Description :

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.

References :

(1) Jerke U, J Biol Chem. 2011. 286(9):7070-81.
(2) Losse J, J Immunol. 2010. 184(2):912-21.
(3) DiScipio RG, J Immunol. 1998. 160(8):4057-66.