Insulin (Ovine) ELISA

Brand

ALPCO

Description

pInsulin (Ovine) ELISA/p
pimg alt=For Research Use Only src=http://www.alpco.com/img/ruo.gif align=absMiddle / For Research Use Only/p
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div class=prod_spec_box
div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
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div class=prod_specSample Type:/div
div class=prod_spec_infoserum, EDTA plasma/div
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div class=prod_specbSample Size:/b/div
div class=prod_spec_info25 micro;l/div
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div class=prod_specbSize:/b/div
div class=prod_spec_info96 wells/div
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div class=prod_spec_box
div class=prod_specbRange:/b/div
div class=prod_spec_info0.15 – 4 ng/ml/div
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div class=prod_specbSensitivity:/b/div
div class=prod_spec_info0.14 ng/ml/div
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div class=prod_specbIncubation Time:/b/div
div class=prod_spec_info2 Hour 15 Min/div
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pbSummary:/b/p
The ALPCO Insulin (Ovine) EIA is a two site enzyme immunoassay. Mouse monoclonal antibodies specific for insulin are immobilized to the 96-well microplate as the solid phase. Standards, samples, and controls are added to the appropriate wells with a horseradish peroxidase enzyme labeled monoclonal antibody (Conjugate), resulting in insulin molecules being sandwiched between the solid phase and Conjugate. After incubation on a microplate shaker at room temperature, the microplate wells are washed with Wash Buffer to remove unbound Conjugate. TMB Substrate is added to each well, and the microplate is again incubated on a microplate shaker at room temperature. During the second incubation, a blue color results from TMB Substrate reacting with bound Conjugate in the wells. Stop Solution is added and this stops the reaction and changes the color from blue to yellow. The optical density (OD) is measured by microplate reader at 450nm with a reference wavelength of 620nm. The intensity of the yellow color is directly proportional to the amount of insulin in the sample.
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Application

Reactivity

Photos