ISG15 Activating Enzyme (E1)

PDF

E-309

25 μg

Brand

Boston Biochem

Description

ISG15 Activating Enzyme (E1)

Catalog #: E-309

ISG15 Activating Enzyme (E1)
Product Source: 

human reconbinant

Also known as: UBA7

This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

Product Information
Quantity: 

25 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 

112 kDa

Purity: 

> 90% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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