Isopeptidase T (USP5), short form

PDF

E-324

25 Î¼g

Brand

Boston Biochem

Description

Isopeptidase T (USP5), short form

Catalog #: E-324

Product Source:

human recombinant

Also known as: IsoT

Isopeptidase T is a member of the high molecular weight ubiquitin C-terminal hydrolases (UCHs) that are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. This enzyme primarily disassembles free polyubiquitin chains linked through isopeptide bonds. This activity has a critical role in the recycling of Ub and in the regulation of the activity of the 26S proteasome. This isoform results from a splicing variant of the long isoform, and differs by a substitution of 23 amino acids (G⁶²⁹-S⁶⁵²) with a single alanine.

Product Information

Quantity:

25 μg

Stock:

X mg/ml (X μM) in 50 mM HEPES, 150 mM NaCl, 1mM DTT, pH 8.0. Actual concentration will vary with specific Lot #.

MW:

95 kDa

Purity:

> 95% by SDS-PAGE

Use & Storage

Use:

Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.