Llama IgG2b Fc Protein, Tag Free
IGB-L5204
1mg
Brand
ACROBiosystems
Description
Source :
Llama IgG2b Fc, Tag Free (IGB-L5204) is expressed from human 293 cells (HEK293). It contains AA Glu 1-Ser 243 (Accession # AAX73259.1).
Molecule : IgG2b Fc
Synonyms : IgG2B,IgG2b Fc
Format : Powder
Category : Other Recombinant Proteins
Accession : AAX73259.1
Storage : -20℃
Shipping condition : Powder,RT
Molecular Weight : 27.3 kDa
Characteristics :
This protein carries no “tag”. The protein has a calculated MW of 27.3 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level : Less than 1.0 EU per μg by the LAL method.
Buffer : Tris with Glycine, Arginine and NaCl, pH7.5
Description :
Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).
References :
(1) Sikorav J.-L., et al., 1980, Nucleic Acids Res. 8:3143-3155.
(2) Ollo R., et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:2442-2446.
Application
Reactivity