Mouse monoclonal antibody to human NGFR/p75NTR [8J2]: IgG 

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR

Antibody_Isotype
(Antibody only) : IgG2a

Application_Details :

Flow Cytometry: 5-20 ug/mL.
Western Blotting: 0.5-2.0 ug/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol).
Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein.
Immunopanning: 1-5 ug/mL.
Immunocytochemistry: 1-5 ug/mL. Staining is strongest in non-fixed cells, light fixation is tolerable.
Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Background_Info :

Clone_Name
(AB only, monoclonals) : 8J2

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1642

Description (Long Description) : 

p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig’s Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.

Expiry_Date : 

12 months after purchase if unopened.

Format (Form) : 

Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, without preservatives.

Immunogen (Antigen) :Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.

Kit_Components :

MSDS (File name) :