Mouse monoclonal antibody to Nestin [4D11] (317-630)

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

Nestin; NES;

Antibody_Isotype
(Antibody only) : IgG

Application_Details :

Western Blotting (WB), Immunocytochemistry (IC) and Flow Cytometry. Suggested dilution for WB is 1:1,000-5,000 and 1:250-500 for IC. Use 2ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Background_Info :

Clone_Name
(AB only, monoclonals) : 4D11

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1011

Description (Long Description) : 

Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240kDa. However the real molecular weight is considerably less than this, at 177kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.

Expiry_Date : 

12 months after purchase

Format (Form) : 

Lyophilized from ascites fluid.

Immunogen (Antigen) :Partial segment (region 317-630 aa) of human Nestin expressed in E.coli

Kit_Components :

MSDS (File name) :