Phospho-Specific Monoclonal Antibody Package (Rat)磷酸化特異性單克隆抗體代製（大鼠)

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Description

GenScript的單克隆抗體生產的雜交瘤細胞株來自骨髓瘤細胞融合和抗體生產B淋巴細胞。 GenScript的創新，包括先進的抗原設計軟件，專有的T MaxTM佐劑，納米技術為抗原製備和國家的最先進的雜交瘤技術，我們的自定義單克隆抗體確保高品質和競爭力的價格。我們的動物設施得到AAALAC的國 際認可。

我們保證為兩個陽性克隆單克隆抗體的開發在任何宿主，如果由GenScript肽抗原設計，合成和共軛。對於任何蛋白抗原（至少2毫克，“50％純度）， 我們保證兩個陽性克隆的Western blot結果。

Phase I: Peptide synthesis. Time: 3-4 weeks.

• We will synthesize 15 mg of phospho-peptide (up to 15 residues) with >90% purity and 10 mg non-phospho-peptide with >70% purity. 5 mg of phospho-peptide and 5 mg of non-phospho-peptide will be delivered to the customer along with the final antibody product.

Phase II: Immunization and ELISA screening. Time: 8-10 weeks.

• Conjugation: The modified peptide is conjugated to a carrier protein, such as KLH.
• Immunization: 10 Balb/c mice are immunized and boosted with a standard immunization protocol.
• Screening: Tail bleedings are screened by ELISA or other method if needed with phospho and non-phospho-peptide.

Phase III: Fusion and screening. Time: 4-8 weeks.

• Fusion: 2 fusions (1-2 spleens per fusion) are performed on the best mice that responded the best to the phospho-peptide in Phase II. Additional fusions are available at an extra charge.
• Screening: The hybridoma cells are screened by ELISA or other method if needed with phospho and non-phospho-petide and all positive clones are expanded into 24-well plates. The clones with best ELISA value and specificity to phospho-peptide are selected for subcloning.

Phase IV: Subcloning, expansion, cryopreservation and delivery. Time: 4-6 weeks.

• Subcloning: Hybridoma subclones are screened by ELISA with phospho and non-phospho-petide. We subclone up to five positive parental clones by limiting dilution, isotype the final positive supernatants, and expand and cryopreserve two subclones for each parental.
• Delivery: Two vials of frozen cells and 5 ml of culture supernatants for a selected subclone. We will gladly provide subcloning for orders involving additional parental clones for an additional fee.

Total Time: 5-7 months.

注意：所有抗血清或純化抗體是以凍乾形式提供的，除非特殊的要求。凍乾延長抗體的壽命，消除了漏水問題，放鬆了嚴格的溫度要求在運輸，減少了運輸過程的總重量。

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