Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P)

Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details

Reactivity

Human, Mouse, Rat

Antibody Type

Polyclonal

Host Species

Rabbit

Immunogen

This antibody was raised against microtubules derived from rat brain.

Formulation

Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

Store between 2°C and 8°C.

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 – 0.5 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.1 – 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC).

This antibody is well characterized and highly reactive to neuron specific Class III β-Tubulin (βIII), but does not identify β-tubulin found in glial cells. This product may contain other non-IgG subtypes.