Rabbit polyclonal antibody to CD40 Ligand (47-65): Affinity purified

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

CD40-L; TRAP; gp39; TRAM; CD154; Tumour necrosis factor ligand superfamily member 5; TNFSF5; CD40L;

Antibody_Isotype
(Antibody only) :

Application_Details :

Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human CD40L has a predicted length of 261 residues and MW of 29 kDa. The observed size is 32 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect CD40L in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Background_Info :

Clone_Name
(AB only, monoclonals) :

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1218

Description (Long Description) : 

CD40L is the ligand for CD40, a member of the tumour necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L. Microglia and astrocytes interact via CD40 receptor and CD40 ligand.

Expiry_Date : 

12 months after purchase

Format (Form) : 

Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3

Immunogen (Antigen) :A synthetic peptide (HRRLDKIEDERNLHEDFVF) corresponding to amino acid sequence 47-65 from the N-terminus of human CD40L.

Kit_Components :

MSDS (File name) :